African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12179

Table of Content: 29 March 2010; 9(13)

March 2010

The isolation and amplification of full length cDNA of oleosins from oil palm (Elaeis guineensis Jacq.)

This study focused on the isolation and amplification of the cDNA clone of oleosin from oil palm. The oil palm cDNA library constructed from the kernel tissues 10 weeks after anthesis (WAA) produced a clone of pO1A containing partial 563 bp sequence of oleosin. The polymerase chain reaction for the rapid amplification of cDNA ends (RACE-PCR) was performed to obtain full length cDNA of oleosin from the RNA transcripts...

Author(s): Shariza Binti Jamek, Ngoh Gek Cheng and Chuah Teon Guan

March 2010

In silico cloning and bioinformatic analysis of PEPCK gene in Fusarium oxysporum

Phosphoenolpyruvate carboxykinase (PEPCK), a critical gluconeogenic enzyme, catalyzes the first committed step in the diversion of tricarboxylic acid cycle intermediates toward gluconeogenesis. According to the relative conservation of homologous gene, a bioinformatics strategy was applied to clone Fusarium oxysporum phosphoenolpyruvate carboxykinase gene (PEPCK) by blasting search of EST database...

Author(s): Li He, Zhou Guoying, Zhang Huai-yun, Li Lin and Liu Jun-ang,

March 2010

Comparative analysis of regulatory elements in different germin-like protein gene promoters

Germin and germin-like proteins (GLPs) the members of cupin superfamily of proteins, which are functionally most diverse proteins. Germin and GLPs have some unique features as they are highly resistant to proteases and to degradation by heat, high pH and detergents like Sodium dodecyl Sulphate (SDS). They are water soluble extracellular enzymatic protein that may also have Oxalate Oxidase (OxO), Superoxide dismutase...

Author(s): Tariq Mahmood, Nazia Nazar, Tayyaba Yasmin, Bilal Haider Abbasi, Mushtaq Ahmad and Syed Muhammad Saqlan Naqvi

March 2010

An improved Agrobacterium mediated transformation in tomato using hygromycin as a selective agent

Bacterial wilt is a devastating disease of tomato crop throughout the world. This disease is very dangerous in hot and humid regions, where it spreads with the irrigation water to whole field within days, which resulted in severe decline in yield. Two varieties of tomato were used for developing bacterial wilt resistance. Riogrande responded more efficiently as compared to Roma. Regeneration frequency of 90.6% was...

Author(s): Zubeda Chaudhry and Hamid Rashid

March 2010

Comparison of transgenic plant production for bacterial blight resistance in Pakistani local rice (Oryza sativa L.) cultivars

The study was carried out to improve bacterial leaf blight resistance in three rice cultivars (Basmati - 370, DR - 82 and IR - 6) by Agrobacterium mediated transformation system. Three week-old scutellum derived calli were infected withAgrobacterium strain EHA101, containing binary vector pTCL5 which has Xa 21 gene. Different levels of acetosyringone were tested to enhance transformation efficiency....

Author(s): M. Z. Rafique, M. Zia, H. Rashid, M. F. Chaudhary and Z. Chaudhry

March 2010

Inheritance of fresh seed dormancy in Spanish-type peanut (Arachis hypogaea L.): bias introduced by inadvertent selfed flowers as revealed by microsatellite markers control

Production and seed quality in peanut (Arachis hypogaea L.) can be reduced substantially by in situ germination under unpredictable rainfed environments. Inheritance of fresh seed dormancy in Spanish x Spanish crosses was studied with two sets of segregating populations, an F2 population derived from true F1 hybrids identified with peanut microsatellites markers and other populations (F2,...

Author(s): Faye Issa, Foncéka Danièl, Rami Jean-François, Tossim Hodo-Abolo, Sall Mbaye Ndoye, Diop A. Tahir and Ndoye Ousmane,

March 2010

Effect of salt stress on germination and early seedling growth of rice (Oryza sativa L.)

The response of twelve rice varieties against six salinity levels (0, 4, 8, 12, 16 and 20 dS m-1) were studied at germination and early seedling stages. Data were analyzed using SAS and means were separated by LSD for final germination percentage (FGP), speed of germination (SG), germination energy percentage (GE%), plumule and radical length and plumule and radical dry weight. Based on dry matter yield reduction, rice...

Author(s): M. A. Hakim, A. S. Juraimi, M. Begum, M. M. Hanafi, Mohd R. Ismailand A. Selamat

March 2010

Bacteria from contaminated urban and hilly areas as a source of polyhydroxyalkanoates production

Polyhydroxyalkanoates (PHA) production and extraction of different bacterial strains isolated from contaminated urban and hilly areas was conducted. The 30 bacterial isolates were Gram negative and belonged to Pseudomonas, Citrobacter, Klebsiella, Escherichia and Enterobacter genera. Bacterial level of resistance against antibiotics (Penicillin) and heavy metals (zinc, cadmium and copper) was...

Author(s): Attia Razzaq, Nazia Jamil, Nighat Naheed and Shahida Hasnain

March 2010

Coculture fermentation of banana agro-waste to ethanol by cellulolytic thermophilic Clostridium thermocellum CT2

Banana is a major cash crop of many regions generating good amount of waste after harvest. This agro waste which is left for natural degradation is used as substrate for single step ethanol fermentation by thermophilic, cellulolytic, ethanologenic Clostridium thermocellum CT2, a new culture isolated from elephant droppings. Scanning electron microscopic pictures clearly indicate cellulolysis and close...

Author(s): Harish Kumar Reddy Y., Srijana M., Madhusudhan Reddy D. and Gopal Reddy

March 2010

Imidacloprid adsorption by soils treated with humic substances under different pH and temperature conditions

The mobility of a pesticide in soil is determined by the extent and strength of sorption, which is influenced by either the existing soil humus or exogenous humic substances. Exogenous humic acids (HAs) were added to soil to enhance the amount of soil organic carbon (SOC) by 2.5, 5.0 and 10.0 g kg-1. Imidacloprid sorption of the treated soils was studied at three pH levels (4.5,...

Author(s): Lifeng Ping, Chunrong Zhang, Yahong Zhu, Min Wu, Fen Dai, Xiuqing Hu, Hua Zhao and Zhen Li

March 2010

The preliminary study of urbanization, fossil fuels consumptions and CO2 emission in Karachi

According to population, Karachi is the first largest city of Pakistan and 9th largest in the world. During last three decades, it has faced mass urbanization, huge population growth, many fold increase in vehicles and industrial development. As a result the demand of more energy in form of fossil fuels increased for domestic, industrial and transportation purpose. In this research the maximum available data of...

Author(s): S. H. Sajjad, N. Blond, A. Clappier, Asif Raza, Safdar Ali Shirazi and Khadija Shakrullah

March 2010

Medium optimization for acarbose production by Actinoplanes sp. A56 using the response surface methodology

In order to improve the production of acarbose, the fermentation medium of acarbose-producing strain Actinoplanes sp. A56 was optimized in this paper. Firstly, fractional factorial design was employed to investigate the influences of glucose, maltose, corn steep liquor, soy bean flour and monosodium glutamate on acarbose production, and the results revealed that maltose and corn steep...

Author(s): Sai-jin Wei, Xin Cheng, Lin Huang and Kun-tai Li

March 2010

Bioconversion of ferulic acid and 4-vinylguaiacol by a white-rot fungus isolated from decaying wood

A white rot fungus isolated from decaying wood was investigated for its ability to convert ferulic acid to various valuable products. The fungus is able to convert ferulic acid to 4-vinylguaiacol, which is then metabolized further to acetovanillone. Both products have potential use in the chemical manufacturing and pharmaceutical industries and these results contribute to our knowledge of the biotransformation of...

Author(s): L. V. Mabinya, T. Mafunga and J. M. Brand

March 2010

Physico-chemical and toxicological studies on Afzelia africana seed and oil

Afzelia africana seeds were obtained from three locations, Abakaliki, Enugu and Nsukka, all in the Eastern part of Nigeria and de-hulled. The seed oil was extracted with (40 - 60°C) petroleum ether and the oil was separated from the solvent using a rotary evaporator. Result of analysis shows that the acid value, saponification value, specific gravity, free fatty acid and refractive index of the oil samples were...

Author(s): P. M. Ejikeme, L. N. Obasi and A. C. C. Egbuonu

March 2010

Construction of a novel lentiviral vector carrying human B-domain-deleted factor VIII gene

Vectors derived from human immunodeficiency virus-1 (HIV-1) are highly efficient vehicles for gene delivery. The present study aimed to establish a potent expression system of human factor VIII (FVIII) with lentiviral vectors. FVIII³BD gene was obtained by enzyme digestion and inserted into lentiviral vector pXZ208 driven by cytomegalovirus (CMV) promoter/enhancer. Recombinant viral particles were prepared by...

Author(s): Hai Cheng, Kailin Xu, Chong Chen and Lingyu Zeng

March 2010

Construction of retroviral recombinant containing human tissue inhibitor of metalloproteinase-2 (TIMP-2) gene and spontaneous invasion of gastric carcinoma cell lines in vitro

Recombinant retroviral vector containing human tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) gene was constructed and investigation of the in vitroinvasion and metastasis of gastric cancer cells transfected with TIMP-2 was carried out. Human TlMP-2 was isolated from recombinant vector Bluescript 1/TIMP-2(+), and then inserted into the retroviral vector pL-MT. Correct orientation was verified by...

Author(s): Nan Li, Xueming Wang, Junshan Zhai, Yanmei Wang, Chaohui Zhu, Lina Sha

March 2010

Manipulation of photoperiod in growth factors of beluga sturgeon Huso huso

The beluga sturgeon is considered as the most important species for caviar production. This study aimed to evaluate effects of photoperiod manipulation on growth factors of beluga sturgeon. The six photoperiod regimes (light: dark cycle) including natural photoperiod (control), 24L: 0D, 16L: 8D, 12L: 12D, 8L: 16D and 0L: 24D with the three replicates. The treatment 5 (8L: 16D) gained the greatest final weight (2194.4 g)...

Author(s): M. R. Ghomi, R. M. Nazari, M. Sohrabnejad, M. Ovissipour, M. Zarei, A. Esmaili Mola, C. Makhdoomi, A. Rahimian, H. Noori and A. Naghavi

March 2010

A simple and rapid nucleic acid preparation method for reverse transcription polymerase chain reaction (RT-PCR) in dormant potato tubers

In order to shorten and facilitate the preparation of nucleic acid (without using tuber slicer, santurugation, vacuum devices and nanocalorimeter (NCM)) for reverse transcription polymerase chain reaction (RT-PCR), pieces of tuber were placeddirectly into eppendorf tubes containing 30 µl of detergent (0.5% Triton XL-80 N)buffers, vortexed for 3 min and then...

Author(s): Hidayet Bostan and Ibrahim Ozer Elibuyuk

March 2010

The effects of different levels of sodium caseinate on rumen fermentation pattern, digestibility and microbial protein synthesis of Holstein dairy cows

Production and seed quality in peanut (Arachis hypogaea L.) can be reduced substantially by in situ germination under unpredictable rainfed environments. Inheritance of fresh seed dormancy in Spanish x Spanish crosses was studied with two sets of segregating populations, an F2 population derived from true F1 hybrids identified with peanut microsatellites markers and other populations (F2,...

Author(s): M. Kazemi-Bonchenari, K. Rezayazdi, A. Nikkhah, H. Kohram and M. Dehghan-Banadaky

March 2010

Identification of retrotransposon-like sequences in Iranian river buffalo

Retrotransposon elements are peculiar genetic elements raised through copy and paste mechanism by retrotransposition. Their ability to move and/or replicate inside the genome is an important evolutionary force responsible for the increase of genome size and the regulation of gene expression. In this paper, molecular identification of retrotransposon-like elements including seven LTR and non-LTR (LINE and SINE) like...

Author(s): Borhan Shokrollahi, Cyrus Amirinia and Navid Dinparast Djadid

March 2010

Studies on hemorrhagic pneumonia in Moschus sifanicus

A series of investigations were carried out including epidemiology, etiology and pathology on hemorrhagic pneumonia in Moschus sifanicus, which had prevailed in Xinglong Mountain National Nature Reserve District in Gansu province of China. The results indicated that the prevalence of this disease could be correlated with local humidity in Xinglong Mountain in Gansu province of China....

Author(s): H. Lu, Y. D. Huang , B. Y. Zhao, J. J. Hu, Q. F. Li and R. W. Dieter 

March 2010

Preliminary pharmacognostical and phytochemical investigation on Sterculia foetida Linn. seeds

Powdered seed of Sterculia foetida (Sterculiaceae) were subjected to successive Soxhlet extraction with chloroform, ethanol and finally with water to get their respective extracts for detailed chemical analysis. Fluorescence character of different extracts and seed powder with various reagents were noted under UV and under normal ordinary light. The total ash value, acid insoluble ash value and water...

Author(s): Shahabadkar G. Shamsundar and Swamy Paramjyothi