August 2012
Construction of a bimolecular fluorescence complementation (BiFC) platform for protein interaction assays in plants
Protein–protein interactions are essential for signal transduction in cells. Bimolecular fluorescence complementation (BiFC) is a novel technology that utilises green fluorescent proteins to visualize protein–protein interactions and subcellular protein localisation. BiFC based on pSATN vectors are a good system for high-level expression of fused protein. A series of pCAMBIA vectors were most...
August 2012
Comparison of lignocellulose composition in four major species of Miscanthus
Miscanthus is a perennial grass rich in lignocellulose that has attracted interest as a non-food crop for renewable bioenergy with major environmental and economic benefits for China. The lignocellulose composition of whole stems of four major species of Miscanthus was assessed. The average values of total moisture content (TMC) (61.90%) and hemicelluloses (34.86%) were the highest while...
August 2012
Influence of kinetin on in vitro rooting and survival of banj oak (Quercus leucotrichophora L.)
A study concerning the influence of cytokinins on shoot regeneration by using different stem segments derived from in vitro raised seedlings and their subsequent rooting was conducted in banj oak (Quercus leucotrichophora L.). Cytokinins play an important role in shoot regeneration and their multiplication. In the present study, cytokinins particularly kinetin (Kn) influenced in...
August 2012
Effect of organic waste compost and microbial activity on the growth of maize in the utisoils in Port Harcourt, Nigeria
One of the major problems of agricultural soils in the coastal areas of the Niger Delta is the low organic matter content. Therefore, land application of composted organic material as a fertilizer source not only provides essential nutrients to plants, it also improves soil quality and effectively disposes soil wastes. In this study, poultry droppings, spent mushroom wastes, earthworm casts and soil samples...
August 2012
Development of sequence characterized amplified region (SCAR) markers linked to race-specific resistance to Striga gesnerioides in cowpea (Vigna unguiculata L.)
An amplified fragement length polymorphism (AFLP) fragment, E-ACT/M-CAA524, tightly linked to the Striga gesnerioides race 1 (SG1) resistance gene Rsg-2-1 in cowpea (Vigna unguiculata L.) was isolated by polyacrylamide gel electrophoresis, cloned, and its nucleotide sequence determined. Based on the resulting sequence information, a pair of sequence specific primers were...
August 2012
Isolation and characterization of a protease-producing thermophilic bacterium from an African hot spring
The aim of this study was to screen for bacterial isolates from a hot spring in Eastern Zimbabwe (conditions in the hot spring: temperature 53 to 54°C and pH 9.3) for organisms of biotechnological interest. Screening of water samples from the hot spring resulted in the isolation of a novel bacterium with interesting protease activity. The bacterium was rod-shaped, Gram negative, motile, non-sporulating...
August 2012
Production of pectinase by Bacillus subtilis EFRL 01 in a date syrup medium
A newly isolated Bacillus subtilis EFRL 01 was used to produce pectinases in batch fermentations on various inexpensive media. A mineral medium based on waste date syrup (15 g/L) as the carbon source and yeast extract (7.5 g/L) as the nitrogen source proved to be the most effective. A 48 h batch fermentation in this medium with a starting pH of 8.0 produced a pectinase titer of ~2,700 U/mL at...
August 2012
Rapid multi-residue method for the determination of pesticide residues in human serum
Exposure to pesticides can represent a potential risk to humans. Agricultural workers are at risk of chronic toxicity. Hence, the evaluation of pesticide residues in their blood gives an indication about the extent of exposure and help in assessing adverse health effects. The aim of our study was to develop analytical method for the simultaneous determination of some residues of pesticides using gas...
August 2012
Propagation and conservation of Dactylorhiza hatagirea (D. Don) Soo, an endangered alpine orchid
Exposure to pesticides can represent a potential risk to humans. Agricultural workers are at risk of chronic toxicity. Hence, the evaluation of pesticide residues in their blood gives an indication about the extent of exposure and help in assessing adverse health effects. The aim of our study was to develop analytical method for the simultaneous determination of some residues of pesticides using gas...
August 2012
Phytochemical properties of some solvent fractions of petroleum ether extract of the African mistletoe (Loranthus micranthus Linn) leaves and their antimicrobial activity
The leaves of the African Mistletoe (Loranthus micranthus Linn) on Kola acuminata in Nsukka, Eastern Nigeria were studied. The chloroform (A), ethyl acetate (B) and ethyl acetate residue (C) fractions of crude petroleum ether extract of L. micranthus Linn were subjected to phytochemical and antimicrobial evaluation. Results reveal the presence of protein in fraction C, whereas...
August 2012
Prevalence of internal parasites in sheep/goats and effective economic de-worming plan at upland Balochistan,Pakistan
Experiments were conducted on sheep and goats during 2006- to 2007 to investigate the prevalence, control and efficacy of anthelmintics against internal parasites at two sites (Loralai and Qila Saifullah) in Balochistan, Pakistan. Faecal samples were collected directly from the rectum of 10- to 20% animals from each flock before the administration of...
August 2012
Effect of ascorbic acid supplementation level to diets of indigenous Venda hens on egg production, hatchability and subsequent productivity of chicks
An experiment was conducted to determine the effect of ascorbic acid supplementation level to the diets of indigenous Venda hens on egg production, hatchability and subsequent productivity of the chicks. The first part of the study determined the effect of ascorbic acid supplementation level to the diets of Venda hens on feed intake, egg production, egg weight, egg content, hatchability and chick...
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