January 2006
Aflatoxin contamination of foods in developing countries: Implications for hepatocellular carcinoma and chemopreventive strategies
Hepatocellular carcinoma (HCC) is the fifth leading cause of cancer mortality in the world and, in certain parts of Asia and Africa, it accounts for about 70% of cancer deaths. Chronic hepatitis B virus (HBV) infection and dietary exposure to aflatoxin B1(AFB1) are the two major risk factors in multi factorial aetiology of HCC. Multiple lines of evidence indicate synergistic interaction between these two agents in...
January 2006
Taxol synthesis
Being a complex diterpenoid, the potent anticancer drug, Taxol, requires complicated steps for its biosynthesis. In the present article, recent advances on Taxol biosynthesis pathway are reviewed, including many recently reported genes that regulate Taxol biosynthesis. To meet the urgent need of clinic and scientific research, besides Taxus supply, other approaches to obtain Taxol have also been discussed...
January 2006
Impregnation and storage of Newcastle disease virus on to filter papers and detection of viral RNA by a single tube RT-PCR assay
Suitability of storing infected allantoic fluid (AF) and cell culture supernatants (CCS) with strain I-2 of Newcastle disease virus (NDV) on Whatman filter papers at room temperature (22-25°C) and 37°C was determined. RNA was extracted from filter papers or liquid aliquots and subjected to reverse transcriptase-polymerase chain reactions (RT-PCR). The results showed that filter papers soaked with NDV...
January 2006
Can a general shoot proliferation and rooting medium be used for a number of carnation cultivars?
This investigation was conducted to find the best shoot proliferation and rooting media for 13 virus-free cultivars of carnation (Dianthus caryophyllus L.) recently imported from The Netherlands to Iran. The best shoot proliferation media were Murashige and Skoog (MS) containing 3 mgL-1 (13.95 mM) kinetin and 0.5 mgL-1 (2.69 mM) NAA or 1 mgL-1 (4.44 mM) BA and 1...
January 2006
Effect of serotonin infusions on the mean plasma concentrations of growth hormone, thyroid hormones and the amount and constituents of milk in the Sanan goat
The goal of this study was to determine whether serotonin increase the mean plasma concentrations of thyroxine (T4), triiodothyronine (T3), growth hormone (GH), milk amount and constituents in the Sanan goats. Nine Sanan goats were randomly divided into 3 groups. Each group received daily infusion of 1, 4 or 8 ng serotonin agonist (hydroxytryptophan) (5HT) for 7 days. Blood and milk samples were collected daily on...
January 2006
Purification and characterization of three low-molecular-weight acid phosphatases from peanut (Arachis hypogaea) seedlings
The maximum acid phosphatasic activity was detected in peanut seedlings the 5th day of germination. At least, three acid phosphatases were identified and purified by successive chromatography separations on DEAE-Sepharose CL-6B, CM-Sepharose CL-6B, Sephacryl S-200 HR, and Phenyl-Sepharose HP to apparent homogeneity from developing five days old peanut seedlings. These enzymes designated acid phosphatase PI, PIIa...
January 2006
Toxicity evaluation of flucycloxuron and diflubenzuron on the cellular model, Paramecium sp.
The effect of two pesticides, the diflubenzuron (DFB) and flucycloxuron (FCX) was studied on the ciliated protiste cellular model, Paramecium sp. Treatment with the DFB at 10 and 20 µg/ml reduces the growth of this protiste appreciably. The survey of the respiratory metabolism by the polarography technique (oxygen electrode) shows a sensitive inhibition of the oxygen consumption. In the case of the FCX,...
January 2006
Nutritional potentials of some tropical vegetable leaf meals: chemical characterization and functional properties
Three tropical vegetable species (Talium triangulare, Amaranthus cruentus and Telfairia occidentalis) were selected based on their availability and agronomic desirability. The freshly harvested vegetable leaves were subjected to 2 processing techniques (shredding and sundrying) before milling into the vegetable leaf meals (VLMs). The crude protein ranged from 19.9+1.8 to 35.1+1.7. The crude fibre ranged from...
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