April 2012
Genetic diversity and relationship analysis of the Brassica napus germplasm using simple sequence repeat (SSR) markers
Oilseed rape (Brassica napus L.) is an important oilseed crop worldwide. The objective of this research was to study the genetic diversity and relationships of B. napus accessions using simple sequence repeat (SSR). A set of 217 genotypes was characterized using 37 SSR markers of mapping on the B. napus genome. The detected alleles were 2 to 11 at each of the 37 markers, with an...
April 2012
Development of bacterial cell-based system for intracellular antioxidant activity screening assay using green fluorescence protein (GFP) reporter
The novel bacterial cell-based assay was developed for evaluating the intracellular antioxidant activity. The genetically engineered Escherichia coli strains harboring the fusions of sodA::gfp and fumC::gfp were constructed and applied as reporters in response to cellular superoxide stress. Using this assay, twelve pure compounds and three Thai medicinal plants were investigated...
April 2012
Construction of mammary gland specific expression plasmid pIN and its expression in vitro and in vivo
The aim of this study was to construct a mammary gland specific expression plasmid pIN and validate its function in expressing goat insulin-like growth factor 1 (IGF-1). The backbone plasmid pBC1 contained goat β-casein 5′ arm and β-casein 3′ arm, to target mammary gland-specific gene expression. First, the igf-1 gene was cloned from liver tissue...
April 2012
A quick DNA extraction protocol: Without liquid nitrogen in ambient temperature
Marker assisted selection is an effective technique for quality traits selection in breeding program which are impossible by visual observation. Marker assisted selection in early generation requires rapid DNA extraction protocol for large number of samples in a low cost approach. A rapid and inexpensive DNA extraction protocol has been described for different tissues of color rice and other plant species...
April 2012
Structure modeling and mutational analysis of gap junction beta 2 (GJB2)
The genome sequencing accomplishes complete genetic blue prints for hundreds of organisms, including humans. In the current era, we are trying to focus on analyzing, controlling and modifying functions of proteins encoded by these genomes. This task is attained by protein three dimensional structures. Three dimensional (3 D) structure is very useful for understanding biological functions. Gap...
April 2012
Sodium nitroprusside (SNP) alleviates the oxidative stress induced by NaHCO3 and protects chloroplast from damage in cucumber
Oxidative damage is often induced by abiotic stress, nitric oxide (NO) is considered as a functional molecule in modulating antioxidant metabolism of plants. In the presentstudy, effects of sodium nitroprusside (SNP), a NO donor, on the phenotype, antioxidant capacity and chloroplast ultrastructure of cucumber leaves were studied under NaHCO3 stress. 30 mM NaHCO3 treatment significantly...
April 2012
Phosphate uptake and growth characteristics of transgenic rice with phosphate transporter 1 (OsPT1) gene overexpression under high phosphate soils
Farmers have used phosphate fertilizer to provide sufficient yields. However, overuse of phosphorus accumulate in soil and causes soil and water pollution. We evaluated the phosphate acquisition and growth characteristics of OsPT1 transgenic rice(OsPT1-OX, over-expressing the high affinity phosphate transporter 1) in high phosphate soils with different level of nitrogen fertilizer...
April 2012
Molecular cloning, structural analysis and expression of a zinc binding protein in cotton
The full-length zinc-binding protein (ZnBP) gene was cloned from a normalized cDNA library constructed from a cotton mutant (Xiangmian-18) during the gland-forming stage. The clone was sequenced and analysed. BLASTP analysis showed that the deduced amino acid sequence of ZnBP in Xiangmian-18 is similar to that inArabidopsis thaliana (GenBank accession no. EFH46337.1) with an overall similarity of 77%....
April 2012
Improving production of laccase from novel basidiomycete with response surface methodology
A Mycena purpureofusca strain screened from six basidiomycota fungi is well characterized in submerged fermentation for its high production of laccase and very low mycelium generation. Optimization of submerged fermentation medium for laccase production by M. purpureofusca was carried out with two statistical methods including Plackett-Burman (P-B) and Box-Behnken (B-B) designs....
April 2012
Partial purification and characterization of polygalacturonase-inhibitor proteins from pearl millet
Polygalacturonase-inhibitor proteins (PGIPs) are plant cell wall glycoproteins, involved in the inhibition of microbial endo-polygalacturonases (EPGs). The present study involved activity guided partial purification of pearl millet [Pennisetum glaucum (L.) R.Br.] protein extract by cation exchange chromatography, which resulted in two pooled protein peaks – Peak-A and Peak-B, both of...
April 2012
Application of high-resolution melting for variant scanning in chloroplast gene atpB and atpB-rbcL intergenic spacer region of Crucifer species
High-resolution melting (HRM) analysis is a rapid and sensitive method for single nucleotide polymorphism (SNP) analysis. In this study, a novel HRM assay was carried out to detect SNPs in the chloroplast gene atpB which encodes the beta subunit of the ATP synthase and atpB upstream intergenic region. The polymorphisms of the two fragments in intertribal samples from the Cruciferae family...
April 2012
Effects of biofertilizers on grain yield and protein content of two soybean (Glycine max L.) cultivars
Nutrient management is one of the most important factors in successful cultivation of plants. Biofertilizers can affect the quality and quantity of crop. In order to study the effects of biofertilizers on grain yield and protein content of two soybean (Glycine max L.) cultivars, an experiment was conducted using a factorial arrangement based on randomized complete block design with four...
April 2012
Construction of mammary gland specific expression plasmid pIN and its expression in vitro and in vivo
The aim of this study was to construct a mammary gland specific expression plasmid pIN and validate its function in expressing goat insulin-like growth factor 1 (IGF-1). The backbone plasmid pBC1 contained goat β-casein 5′ arm and β-casein 3′ arm, to target mammary gland-specific gene expression. First, the igf-1 gene was cloned from liver tissue...
April 2012
Studies on antioxidant capacity of anthocyanin extract from purple sweet potato (Ipomoea batatas L.)
The radical scavenging effects by α,α-diphenyl-β-picrylhydrazyl (DPPH) and superoxide anions of anthocyanin extract from purple sweet potato were investigated. The antioxidation experiments showed that the reducing power of the anthocyanin extract reduced 0.572 at 0.5 mg/ml, while those of L-ascorbic acid (L-AA) and butylated hydroxytoluene (BHT) reduced 0.460 and 0.121, respectively. They...
April 2012
Study on the triphenyl tetrazolium chloride–dehydrogenase activity (TTC-DHA) method in determination of bioactivity for treating tomato paste wastewater
A quick analysis of the sludge activity method based on triphenyltetrazolium chloride-dehydrogenase activity (TTC-DHA) was developed to change the rule and status of the biological activity of the activated sludge in tomato paste wastewater treatment. The results indicate that dehydrogenase activity (DHA) can effectively facilitate the biochemical reaction of tomato paste wastewater treatment upon analysis of...
April 2012
Isolation and characterization of nitrogen fixing bacteria from raw coir pith
Coir fibre is the hard fibre extracted from the coconut husk and coir pith is a lignocellulosic byproduct released during the extraction of coir fibre. The pith is not degraded under normal environmental conditions and accumulates in the fibre extraction units occupying sprawling space in the units. The inherent properties of coir pith make it useful as a plant nutrient. Nitrogen fixing bacteria...
April 2012
Salt-induced osmotic stress for lipid overproduction in batch culture of Chlorella vulgaris
Effect of NaCl-induced osmotic stress on lipid production was investigated in batch culture of Chlorella vulgaris. Based on the facts that NaCl stress improved lipid production but inhibited cells growth at the same times, the novel strategies of multiple osmotic stresses with different NaCl additions (2 g/L at 80 h, 4 g/L at 100 h, and 6 g/L at 120 h) were adopted...
April 2012
Production and partial characterization of an exopolysaccharide from Ustilago maydis in submerged culture
In Mexico, corn is widely cultivated and frequently parasitized by Ustilago maydis, abasidiomycete dimorphic fungus that causes the disease known as corn smut. It iswidely consumed and is considered a culinary delight. From a biotechnologicalperspective, U. maydis offers the possibility as a producer...
April 2012
Study of the effect of PAPA NONOate on the rate of diabetic wound healing
To investigate the effect of exogenous nitric oxide donor (PAPA NONOate) a drug which spontaneously release nitric oxide on the rate of wound healing and collagen synthesis on impaired wound healing in experimental diabetes. 12 male Sprague – Dawley rats were transferred to separate metabolic cages. Nine days before wounding, the rats were injected intraperitoneally (IP) with streptozotocin (STZ) (55...
April 2012
L-Monomethyl-arginine decreases apoptosis of chondrocytes by altering Bax and Bcl-2 expression in osteoarthritis of rabbit knee
Previous studies found that NG-monomethyl-L-arginine (L-NMMA) treatment inhibits progression of osteoarthritis. Here, we aimed to explore the effects of L-NMMA on chondrocyte apoptosis and Bax and Bcl-2 mRNA expression in rabbits with knee osteoarthritis. Knee osteoarthritis was induced in 24 healthy rabbits by Hulth method, and rabbits were randomly divided into control (n = 12) and...
April 2012
Characterization of diploid and triploid Heterobranchus bidorsalis using morphometric, meristic and haematological parameters
The study investigates comparative changes in morphometric, meristic and haematological values of triploid and diploid strain of Heterobranchus bidorsalis with a view to establishing differences and comparative adaptability between the two strains. The experiment was carried out inside net hapas submerged inside 1 × 1 × 1.2 m2concrete tank where diploid (2n) and triploid (3n) fish were...
April 2012
Ultramicromorphological observation of Usnea longissima Ach.
The Usnea longissima Ach. grew as an epiphyte on Abies georgei and was collected at an altitude of 3640 m above sea level from the Pudacuo National Park in the Shangri-La County of the Diqing Tibetan Autonomous Prefecture in the Yunnan Province of China. Scanning electron microscopy revealed the ultramicro-organisation of the U. longissima. We observed that the interior of the...
April 2012
Changes in some biochemical parameters of kidney functions of Plasmodium berghei infected rats administered with some doses of artemether
This study aimed at determining changes in urine concentrations of sodium (Na+) and potassium (K+) of Plasmodium berghei infected rats during a week of intramuscular administration of artemether (12.5 to 50.0 mg/kg/day) and one week thereafter. Their concentrations and that of creatinine and urea in the plasma were also determined at the end of the study. The observed changes were related to the...
April 2012
Salt effect on physiological, biochemical and anatomical structures of two Origanum majorana varieties (Tunisian and Canadian)
In this study, we evaluated the salt concentration effect on plant growth, mineral composition, antioxidant responses and anatomical structure of two varieties ofOriganum majorana after exposure to NaCl treatment. Our results show an inclusive behaviour of the two varieties, since the majority of sodium was exported and accumulated in their aerial parts. The Canadian variety (CV) appeared...
April 2012
Some chemical properties of oil palm decanter meal
The aims of this study were to investigate the rancidity and chemical properties of oil palm decanter meal (OPDM) after been kept over an extended period of time.Samples were collected daily and analyzed for some rancidity properties, including peroxide value (PV) and thiobarbituric acid (TBA), and for chemical composition, including proximate analysis, fiber, mineral and fatty acid (FA) content. The...
April 2012
Immune responses of pigs inoculated with a recombinant fowlpox virus coexpressing ORF2/ORF1 of PCV2 and P1 2A of FMDV
A recombinant fowlpox virus (rFPV-ORF2ORF1-P12A) containing the open reading frame ORF2)/ORF1 DNAs of the porcine circovirus 2 (PCV2) (strain, Inner Mongolia) and foot-and-mouth disease virus (FMDV) capsid polypeptide of O/NY00 was evaluated for its abilities to induce humoral and cellular responses in piglets. In addition, we examined its abilities to protect cell cultures against a homologous virus...
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