African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 3 December 2007; 6(23)

December 2007

Teratogenic effect of isotretinoin on the morphology and palate development in rat fetuses

The effect of isotretinoin currently used in dermatological treatments was investigated on the morphology and palate development during organogenesis in rat fetuses. This was in an attempt to evaluate the morphological implications associated with isotretinoin consumption. The animals were randomly divided into groups: A, B and C. Group A was further divided into subgroups: A1 and A2 and administered orally with...

Author(s): Ofusori, D. A., Adelakun A. E., Jimoh, S. A., Komolafe A.O, Falana B. A., and Abayomi T. A.

December 2007

Phytochemical and antimicrobial screening of the crude petroleum spirit and methanol extracts of the stem bark, leaves and roots of Ficus thoningii (blume)

Ficus thoningii which has some traditional medicinal uses was investigated. Phytochemical screening of the stem bark, leaves and roots gave positive results for carbohydrates, glycosides, saponins and alkaloids. Antimicrobial screening of the crude petroleum spirit and methanol extracts showed activity against Klebsiella pneumoniae, Staphylococcus aureus, Escherichia coli, Providencia...

Author(s): Ndukwe, I. G., Bello, A. I., Habila, J. D. and John, C.

December 2007

Antibacterial properties of Passiflora foetida L. – a common exotic medicinal plant

Passiflora foetida L. (stinking passion flower) is an exotic medicinal vine. The antibacterial properties of leaf and fruit (ethanol and acetone) extracts were screened against four human pathogenic bacteria i.e. Pseudomonas putida, Vibrio cholerae, Shigella flexneri and Streptococcus pyogenes by well-in agar method. The results showed the leaf extract having remarkable activity against all...

Author(s): C. Mohanasundari, D. Natarajan, K. Srinivasan, S. Umamaheswari and A. Ramachandran

December 2007

Structural and functional analysis of the promoters of the human glycerol kinase gene family

During spermatogenesis the X-chromosome is inactivated, but several glycolytic cycle enzymes have been found to have spermatogenic cell specific isoforms encoded by the X-chromosome counterpart genes located on the autosomes. Here, the promoters of the human glycerol kinase gene family were cloned: the somatic gene called GK-Xp, and the two testis-specific isoforms called GK-1 and GK-2,...

Author(s): Banu Az-Zubair, M. K.

December 2007

SSR markers reveal genetic variation between improved cassava cultivars and landraces within a collection of Nigerian cassava germplasm

Thirty-one improved cultivars and five Nigerian landraces of cassava were assessed at genomic DNA level with 16 SSR primers for genetic diversity study. The minimum number of SSR primers that could readily be used for identification of the 36 cassava genotypes was also determined. For the genetic diversity study, the similarity coefficients generated between improved cultivars and Nigerian landraces ranged from 0.42 to...

Author(s): O. K. Moyib, , O. A.  Odunola and A. G. O. Dixon

December 2007

Isolation, biochemical and molecular characterization of 2-chlorophenol-degrading Bacillus isolates

Pure cultures of 2-chlorophenol degrading bacteria were isolated from a natural enrichment that may be adapted to chlorophenols in the industrial zone at Umm-Saied city (Qatar). The bacteria were identified by 16S rDNA analysis, using PCR with universal primers. Comparative analysis of the 16S rDNA sequence (~ 550 bp) in the GenBank database revealed that these bacteria are related to the...

Author(s): Roda F. Al-Thani, Desouky A.M. Abd-El-Haleem, and Mona Al-Shammri

December 2007

Comparative analysis of inter population genetic diversity in Puntius filametosus using restriction fragment length polymorphism (RFLP) analysis

The genetic variation in different population of the freshwater cyprinid Puntius filamentosus was studied using restriction fragment length polymorphism (RFLP) analysis. Samples were collected from five different locations of southern Western Ghats, India. The morphometric characters of population from Alancholai showed little variation when compared with other population. The genomic size of the different...

Author(s): Jeyaraj Antony Johnson, Rajayanian Pusphabai Rajesh, Lidwin Anna Maryand Muthukumarasamy Arunachalam  

December 2007

Induced propagation of African clariid catfish, Heterobranchus bidorsalis (Geoffrey Saint Hillarie, 1809) using synthetic and homoplastic hormones

Induced spawning of African giant catfish (Heterobranchus bidorsalis) was successfully carried out using synthetic hormone (Ovaprim) and natural hormone (homoplastic hormone-pituitary extract from H. bidorsalis). The study which was carried out at Aquafish Farm, Ihiala, Anambra State, Nigeria, lasted 70 days (May to July). Sixty gravid females and twenty mature males of H. bidorsalis (weight...

Author(s): Nwokoye, Charles Ononuju, Nwuba, Lucy Afuluenu and Eyo, Joseph Effiong

December 2007

Delivery of thermostable Newcastle disease (ND) vaccine to chickens with broken millet grains as the vehicle

The efficacy of treated broken millet grains as a carrier for delivery of thermostable Newcastle disease (ND) vaccine HRV4 to free-range chickens in three locations was assessed by haemagglutination inhibition (HI) test and challenge experiment. Out of 256 birds fed with first dose of the vaccine, 130 (50.8%) produced detectable HI antibody but only 16 (6.3%) attained serum antibody level of log2 ≥ 3.0...

Author(s): Echeonwu, G. O. N., Iroegbu, C. U., Echeonwu, B. C., Ngene, A., Olabode, A. O., Okeke, O. I., Ndako, J., Paul, G., Onovoh, E. M., Junaid, S. A. and Nwankiti, O.

December 2007

The utilization of the response surface methodology for the optimization of cultivation medium and growth parameters in the cultivation of the yeast strain S.cerevisiae 3.20 on ethanol

A mutant strain of the yeast Saccharomyces cerevisiae growing on ethanol as single source of carbon and energy was used in optimization experiments at laboratory and micropilot scale, following the surface response methodology. The cultivation medium optimization was performed on the basis of maximization of dry cell weight and the process parameters optimization on the basis of substrate yield...

Author(s):   O. Popa, Narcisa Babeanu, A. Vamanu and E. Vamanu

December 2007

Seasonal and spatial root biomass and water use efficiency of four forage legumes in semiarid northwest China

A field study was conducted to determine seasonal root biomass, root spatial distribution and water use efficiency (WUE) in milkvetch (Astragalus adsurgens Pall.), sainfoin (Onobrychis viciaefolia Scop.), alfalfa (Medicago sativa L.) and Lespedeza davurica (L. davurica) grown in semiarid region on the Loess Plateau, northwestern China. Soil core method (Ø9 cm) was used to...

Author(s): Bingcheng Xu, , Lun Shan, Fengmin Li, and Jun Jiang

December 2007

Immobilization of starch phosphorylase from seeds of Indian millet (Pennisetum typhoides) variety KB 560

Starch phosphorylase has been isolated from the seeds of millet (Pennisetum typhoides) variety KB560 and partially purified using ammonium sulfate fractionation. The partially purified enzyme was desalted using Sephadex G-25 chromatography. In the direction of polysaccharide synthesis, the enzyme showed optimum activity at pH 6.0 with two half pH optima at pH 5.4 and pH 7.0 whereas in the direction of...

Author(s): Sarika Garg and Anil Kumar

December 2007

Oxidative stability and bacteriological assessment of meat from broiler chickens fed diets containing Hibiscus sabdariffa calyces

Thigh muscles were obtained from a batch of 120 broiler chickens fed diets containing 0, 1.5, 3.0, 4.5 and 6.0% dried Hibiscus sabdariffa calyces (diets A, B, C, D and E respectively). The thigh muscles were studied as uncooked and steam-cooked, and refrigerated for up to 9 days. Oxidative stability of muscle was measured using the thiobarbituric acid test and bacteria isolates were identified and quantified....

Author(s): Gbenga Emmanuel Onibi and Innocent Bamidele Osho