February 2011
Analysis of DNA methylation variation in sibling tobacco (Nicotiana tabacum) cultivars
Amplified fragment length polymorphism (AFLP) and methylation-sensitive amplification polymorphism (MSAP) analysis were used to investigate the genome of two sibling tobacco cultivars, Yunyan85 and Yunyan87, their parent K326 and the other tobacco cultivar NC89. AFLP analysis indicated that, the genome primary structure variations were not obviously detected among Yunyan85 and Yunyan87 and K326. However, the...
February 2011
Association between GH encoding gene polymorphism and semen characteristics in Iranian Holstein bulls
The objective of this present study was to investigate relationships between the growth hormone gene restriction fragment length polymorphism (RFLP) and bull sperm characteristics. A total of 89 bulls from two semen evaluation stations were genotyped for the bovine growth hormone (bGH)-AluI polymorphism by polymerase chain reaction and followed by 4% metaphor agaros gel electrophoresis. The overall allele...
February 2011
Time related total lactic acid bacteria population diversity and dominance in cowpea-fortified fermented cereal-weaning food
The total lactic acid bacterial community involved in the spontaneous fermentation of malted cowpea fortified cereal weaning food was investigated by phenotypically and cultivation independent method. A total of 74 out of the isolated 178 strains were Lactobacillus plantarum, 32 were Pediococcus acidilactici and over 60% wereLactobacillus strains. L. plantarum and P....
February 2011
The use of reproductive vigor descriptors in studying genetic variability in nine Tunisian faba bean (Vicia faba L.) populations
A collection of nine Tunisian faba bean (Vicia faba) populations belonging to three botanical classes (Var. minor, var. equina and var. major) was evaluated using twenty seven agro-morphological traits. Analysis of variance, correlation coefficients and principal components analysis (PCA) were performed based on MVSP 3.13 program. Significant differences between populations were noted for most...
February 2011
Virulence properties and random amplification of polymorphic DNA (RAPD) fingerprinting of Candida albicans isolates obtained from Monastir dental hospital, Tunisia
Genotypic and phenotypic characterization as well as studies on the virulence factors of Candida albicans isolates obtained from oral cavity of patients was carried out using random amplified polymorphic DNA (RAPD) fingerprinting and epithelial cells adherence assay, respectively. RAPD patterns revealed the presence of 13 C. albicans genotypes separated into two clusters at...
February 2011
Genetic relationships between some released and elite Ghanaian cassava cultivars based on distance matrices
Eleven (11) released and two local Ghanaian cassava cultivars were fingerprinted to estimate the genetic diversity among them using 35 SSR markers. Genomic DNA of thirteen cassava cultivars (UCC, IFAD, Agelifiaa, Nyerikobga, Nkabom, Essam Bankye, Akosua Tumtum, Debor, Filindiakong, Afisiafi, Doku Duade, Bankye Hemaa and Bankye Botan) were isolated and used as template for PCR amplification...
February 2011
Morphological characterization of the local potato (Solanum tuberosum L.) genotypes collected from the Eastern Black Sea region of Turkey
In this study, 58 villages were selected and a total of 146 samples were taken according to stratified sampling system. These genotypes were collected from production areas in high altitudes of the province of Artvin (41°10’ 54”– 40° 49’ 09” N and 42° 21’ 49”- 41° 32’ 40” E) and Rize (41° 02’43”-40o 46’ 50”...
February 2011
Compatibility of entomopathogenic fungi with extracts of plants and commercial botanicals
The compatibility of some commercial botanicals (Biospark, Phytophrate, Exodos, Biodos and Neemgold) and of solvent extracts of Syndrella nodiflora, Premna tomentosa, Vitex negundo, Ipomea carnea, Pteridium aquilinum (leaves) andAnnona squomosa (seeds) with Beauveria bassiana (Bals.) Vuil., Isaria (Paecilomyces) fumosorosea (Wize) Brown et Smith...
February 2011
Genomics analysis of genes expressed reveals differential responses to low chronic nitrogen stress in maize
A growth system was developed where N was the only growth-limiting factor. Whole-genome transcription profiling of leaf tissues were analyzed using the Affymetrix GeneChip. 129 clones showed significant change and 83 clones were classed accurately. Most induced clones were largely involved in various metabolism processes including physiological process, organelle regulation of biological...
February 2011
Effects of different rhizosphere ventilation treatment on water and nutrients absorption of maize
The objective of this study was to explore the effects of different rhizosphere ventilation treatment on water and nutrients absorption of maize. The pot experiment was conducted using three methods: no ventilation, two day ventilation and four day ventilation, under conditions of the different levels of irrigation methods. As such, the influence of rhizosphere ventilation treatment on the physiological,...
February 2011
Evaluation of iodine content of some selected fruits and vegetables in Nigeria
In the past few decades, there has been renewed interest on increase in intake of fruits and vegetables, owing to their numerous beneficial effects. The present study provides preliminary data on the ability of different fruits and vegetables grown and consumed in Ijebu North Local Government Area of Ogun State, Nigeria to concentrate iodine in their tissues, given the same environmental conditions. Chemical...
February 2011
Detecting bacterial endophytes in tropical grasses of the Brachiaria genus and determining their role in improving plant growth
Plant-growth-promoting (PGP) bacteria include a diverse group of soil bacteria thought to stimulate plant growth by various mechanisms. Brachiaria forage grasses, of African origin, are perennials that often grow under low-input conditions and are likely to harbour unique populations of PGP bacteria. Three bacterial strains that tested positive for nitrogenase reductase gene sequences (nifH) were...
February 2011
Comparison of the adhesion ability of Candida albicans strains to biotic and abiotic surfaces
The purpose of the present study is to investigate the ability of oral Candida albicans strains to adhere to Caco-2 and Hep-2 epithelial cells, to produce slime using Congo red and Safranin methods and to form a biofilm on polymethylmethacrylate. A total of 20 C. albicans strains were tested in the present work. The biofilm formed by C. albicans isolates on acrylic denture...
February 2011
Antifungal and antibacterial effects of some acrocarpic mosses
In this study, the antifungal and antibacterial effect of 6 different acrocarpous mosses were tested in vitro aganist 8 different microorganisms. For the extraction, ethyl alcohol, methyl alcohol, acetone and chloroform were used as solvents. While the highest antimicrobial effect was seen in methyl alcohol extracts, extracts of chloroform showed the lowest level of antimicrobial...
February 2011
Evaluation of anticancer activity of Debregeasia Salicifolia extract against estrogen receptor positive cell line
Crude methanol extract and fractions of Debregeasia salicifolia stem were examined for their anticancer activity. To determine anticancer activity, different concentrations of crude extract were tested on MCF-7 cancer cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. D. salicifoliaextract showed a significant antiproliferative activity and a dose...
February 2011
Identification and expression analysis of vitellogenin from silk-producing insect, Actias selene Hubner
This study mainly shows the DNA sequence and expression analysis of vitellogenin in Actias selene (Ash-Vg). Specific primers were designed to amplify Ash-Vg gene by polymerase chain reaction (PCR) and the obtained DNA sequence was 7329 bp long, including 6 exons and 5 introns with an open reading frame encoding a 1774 amino acids peptide. A Bm DSX binding site and some conserved signatures such as...
February 2011
Assessing hygienic behavior and attraction to Varroa mite (Acari: Varroidae) in Iranian honey bee (Apis. mellifera meda)
In the current study, the hygienic behaviors of 5th instar larva of Iranian honeybees (Apis mellifera. meda) were investigated. The results of hygienic evaluation demonstrated that 35% of Iranian honeybees are hygienic. For more research, different levels of hygienic behaviors were used as a treatment and then the selected 5th instar larva were transferred to fundamental colonies with 10 to 12 %...
February 2011
Construction of PVX virus-expression vector to express enterotoxin fusion gene LTB-ST and transformation into tobacco
Potato X potyvirus (PVX)-based vector has been comprehensively applied in transient expression system. In order to produce the heterologous proteins more quickly and stably, the ClaI and NotI enzyme sites were introduced into the Enterotoxin fusion gene LTB-ST by polymerase chain reaction (PCR) and the LTB-ST gene was introduced into the PVX-based vector in the site of...
February 2011
Enhanced xanthan production process in shake flasks and pilot scale bioreactors using industrial semi-defined medium
Xanthan gum, a heteropolysaccharide produced by Xanthomonas campasteris, is a widely used biopolymer in food industries. The production process is highly influenced by the type and concentration of the different carbon and nitrogen source as well as other medium components. The aim of this work was to develop an economic industrial medium for industrial production. In shake flask level, the maximal...
February 2011
Blood glucose lowering effect of aqueous extract of Graptophyllum pictum (Linn) Griff. on alloxan-induced diabetic rats and its acute toxicity in mice
This study was aimed at evaluating the claimed anti-diabetic property of the aqueous extract of Graptophyllum pictum leaf and to establish an effective dose for the extract. It also attempted to unravel if the extract could be acutely toxic to mice. The anti-diabetic study was carried out on alloxan-induced diabetic Wistar rats. After diabetic induction, the rats were divided into 5 groups....
February 2011
Association between milk protein polymorphism and milk production traits in Black and White dairy cattle in Turkey
Milk protein polymorphism such as as1-casein (as1-Cn), b-lactoglobulin (b-Lg), b-casein (b-Cn) and k-casein (k-Cn) of Black and White dairy cattle were investigated in this study. The genetic structure of herd was examined for these loci. At the sametime the relationships between milk protein types and some milk production traits were determined. Milk production traits were chosen as milk...
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