African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12184

Table of Content: 7 May, 2014; 13(19)

May 2014

Loop-mediated isothermal amplification (LAMP) based detection of bacteria: A Review

Various diseases are caused by pathogenic bacteria and their diagnosis depends on accurate detection of pathogen from clinical samples. Several molecular methods have been developed including PCR, Real Time PCR or multiplex PCR which detects the pathogen accurately. However, every method has some limitations like low detection limit, whereas Loop-mediated isothermal amplification (LAMP) is a powerful and novel nucleic...

Author(s): Pooja Saharan, Sudesh Dhingolia, Poonam Khatri, Joginder Singh Duhan and Suresh Kumar Gahlawat

May 2014

Advances in highly specific plant gene silencing by artificial miRNAs

Endogenous microRNAs (miRNAs) are potent negative regulators of gene expression in plants and animals. Through artificially transformed miRNA (amiRNAs) to target one or several genes of interest is becoming a powerful tool for silencing genes. The characteristics and application prospect of artificial microRNA (amiRNA) technology were reviewed.   Key words: Gene-silence, RNA interference, MiRNA,...

Author(s): Guangyu Luo, Ting Su, Cuifang Liu, Jie Zou, Ailing Liu and Xinbo Chen

May 2014

Indigenous leafy vegetables (imifino, morogo, muhuro) in South Africa: A rich and unexplored source of nutrients and antioxidants

South Africa is endowed with agro-biodiversity that consists of different types of indigenous leafy vegetables (ILVs) with health benefits and rich source of nutrients to cater for over three million people suffering from hunger and malnutrition in the country. Unfortunately, the use of these vegetables is declining at an alarming rate due to negligence and lack of appropriate cultivation practices to improve yield,...

Author(s): Njume, C., Goduka, N. I. and George, G.

May 2014

Random amplified polymorphic DNA (RAPD) based assessment of genetic relationships among some Zimbabwean sorghum landraces with different seed proanthocyanidin levels

Knowledge of genetic distances between genotypes is important for efficient organization and conservation of plant genetic resources for crop improvement programs. In this study genetic distances between genotype pairs (complements of Jaccard's similarity coefficient) were estimated from Random Amplified Polymorphic DNA (RAPD) data collected from 48 Zimbabwean sorghum landraces. These varieties showed variation in...

Author(s): Z. Dhlaminiand I. Sithole-Niang

May 2014

Genotypic frequency of calpastatin gene in lori sheep by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method

Calpastatin is a natural occurring inhibitor of calpastatin (CAST) and consequently the balance of calpain-calpastatin activity in muscles is believed to dictate the rate of tenderization in post-mortem meat. Genomic DNA was extracted from 100 sheep blood sample. Polymerase chain reaction was performed to amplify a 622 bp fragment of this gene. Restriction reaction of polymerase chain reaction (PCR) products was done...

Author(s): Nematollah Asadi, Sh. Nanekarani and S. Khederzadeh

May 2014

Effect of priming on germinability and salt tolerance in seeds and seedlings of Physalis peruviana L.

In some species, pre-germination treatments such as priming can increase germinability and the speed of the process, besides conferring tolerance to abiotic stress. The central effect of priming is the slow and controlled absorption of water in seed tissues, allowing the membranes to reorganize and synthesize protective substances against stress. This study was performed to assess the effects of priming on the...

Author(s): Cíntia Luiza Mascarenhas de Souza, Manuela Oliveira de Souza, Lenaldo Muniz de Oliveira and Claudinéia Regina Pelacani

May 2014

Effect of nodal positions, seasonal variations, shoot clump and growth regulators on micropropagation of commercially important bamboo, Bambusa nutans Wall. ex. Munro

An efficient protocol for in vitro micropropagation of Bambusa nutans Wall. ex. Munro has been described. Nodal explants obtained from 1½-year-old field-grown culms of B. nutans produced up to 7.0 multiple shoots per explant on Murashige and Skoog (MS) basal medium supplemented with 6- benzylaminopurine (BAP, 1.0 mg/L). Continuous shoot proliferation up to 11.33 shoots was achieved by sub-culturing shoot clumps...

Author(s): Kalpataru Dutta Mudoi, Siddhartha Proteem Saikia and Mina Borthakur

May 2014

Chemical characterization and local dispersion of slag generated by a lead recovery plant in Central Mexico

A toxic waste “slag” generated in a lead (Pb) recovery plant was characterized and its local “slag” dispersion was monitored. The major constituents of the “slag”, in increasing weight percent, were CaO (1.5), PbO (3.4), SiO2 (5.2), Na2O (17.8), SO3 (27.5) and Fe2O3 (40.7). The “slag” was alkaline with a pH of 10 and an electrical conductivity of 606 dS m-1. Tests for Pb...

Author(s): Barcos-Arias Milton, Vázquez Martínez Juan, Maldonado Vega María, Alarcón Alejandro and Peña-Cabriales Juan José

May 2014

Assessment of the phytoremediation potential of Panicum maximum (guinea grass) for selected heavy metal removal from contaminated soils

Non-vascular plants have potential for rapid uptake of metals, but are rarely used for phytoremediation because of their short life cycle. This property can however be advantageously used in a number of metal removal cycles within a short time. The selection of promising plants is critical to success of phytoremediation. The potential for heavy metal uptake by Panicum maximum a non-vascular plant was investigated using...

Author(s): Olatunji, O. S., Ximba, B. J., Fatoki, O. S. and Opeolu, B. O.

May 2014

Application of mixed models for the assessment genotype and environment interactions in cotton (Gossypium hirsutum) cultivars in Mozambique

In the process of introducing cotton cultivars, it is essential to assess their productive behavior for different environments for which they will be recommended. Knowledge of the magnitude of the genotype interaction with environment allows the evaluation of the stability and adaptability of genotypes where one intends to introduce them, in addition to enabling the evaluation of the production potential and possible...

Author(s): Leonel Domingos Moiana, Pedro Soares Vidigal Filho, Maria Celeste Gonçalves-Vidigal, Manuel Pedro Maleia and Noimilto Mindo

May 2014

Influence of processing methods on mycoflora changes during storage of raw and processed Atlantic horse mackerel (Trachurus trachurus)

Study assessed the influence of processing on mycoflora of kote fillet, skin, head and bones (SHB) during storage for 28 days at ambient temperature of (32 ± 2°C). Fish samples were prepared by smoking (wood and coal) and poaching using standard methods. Fungi associated with raw and processed fillets and SHBs included the species of Absidia glaucus. Absidia, Aspergillus flavus, Aspergillus niger,...

Author(s): Olaluwa T. Adeyemi, Odutola, Osilesi, O. O. Adebawo, F. D. Onajobi, Sunday O. Oyedemi and Anthony J. Afolayan