African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12359

Table of Content: 7 September; 10(51)

September 2011

Probability to produce animal vaccines in insect baculovirus expression system

  The insect baculovirus expression system is a valuable tool for the production of vaccine. Many subunit vaccines have been expressed in this system. The first vaccine produced in insect cells for animal use is now in the market. In this study, we reviewed recent progress of animal’s vaccine production for different expression levels and baculovirus genome stability, characteristic features of...

Author(s): Bhaskar Roy, Shuang Liang, Peng Zhang, mei-xian Wang, Fang Zhou, Wan Chi Cheong David and Yun-gen Miao

September 2011

The role of synthetic growth hormones in crop multiplication and improvement

  Crop improvement through conventional methods to provide food security for the ever growing population has several limitations. Modern plant biotechnology has held promise over the years to improve outputs from plants. The use of growth hormones as a way of improving plant yield through micro propagation and somatic embryogenesis is the focus of this paper. Improved and disease resistant crops could easily be...

Author(s): Gana, A. S.

September 2011

Advances on research epigenetic change of hybrid and polyploidy in plants

  Hybridization between different species, and subsequently polyploidy, play an important role in plant genome evolution, as well as it is a widely used approach for crop improvement. Recent studies of the last several years have demonstrated that, hybridization and subsequent genome doubling (polyploidy) often induce an array of variations that could not be explained by the conventional genetic...

Author(s): Jian Gao, Zhiming Zhang, Luo Mao, Qin Cheng, Li Liu, Haijian Lin, Yaou Shen, Shibin Gao, Maojun Zhao and Guangtang Pan

September 2011

Population genetic structure analysis in endangered Hordeum vulgare landraces from Tunisia: Conservation strategies

  Genetic markers have been employed in combination with morphologicalcharacters to identify patterns of population structure in 13 barley landrace populations from Tunisia. These endangered barley populations are grown by few local farmers in low-input farming systems. Based on 117 random amplified polymorphic DNA markers and 34 morphological traits, variance analyses indicated that most of...

Author(s): Néjia Zoghlami#, Aida Bouagila#, Myriame Lamine, Haifa Hajri and Abdelwahed Ghorbel

September 2011

Molecular research on the genetic diversity of Tunisian date palm (Phoenix dactylifera L.) using the random amplified microsatellite polymorphism (RAMPO) and amplified fragment length polymorphism (AFLP) methods

  The genetic diversity organization and evaluation of relationships within Tunisian date-palm cultivars were assessed using the random amplified microsatellite polymorphism (RAMPO) and amplified fragment length polymorphism (AFLP) methods. 18 combinations of random/ISSR primers and six AFLP primers combinations were tested with DNAs isolated from 40 date-palm cultivars. Our results...

Author(s): Rhouma-Chatti Soumaya, Baraket Ghada, Dakhlaoui-Dkhil Sonia, Zehdi-Azouzi Salwa and Trifi Mokhtar

September 2011

Molecular cloning and sequence analysis of the cat myostatin gene 5´ regulatory region

  Cat is an important experimental and pet animal and plays a key role in keeping ecological balance. Myostatin is a negative regulator of skeletal muscle growth and development in mammals, but the gene sequence of it keeps unknown in cat. To better understand the structure and function of the myostatin promoter in cat, a 1409 bp fragment containing the 5'-regulatory region of the cat myostatin gene was...

Author(s):   Rong Du, Juan Du, Jian Qin, Li-Chun Cui, Jian Hou, Hong Guan and Xiao-Rong An

September 2011

Origin and differentiation of a special fragment from Capra hircus agouti gene

  A 136 bp fragment previously considered to only exist in Capra hircus agoutigene was analyzed. The results indicated that it existed in the agouti gene ofC. hircus and Ovis aries and in other genes of O. aries and Bos taurus with forward and reverse sequences. The reverse sequences from O. aries had higher genetic diversity than forward ones. The...

Author(s): Weiwei Wu, Xianglong Li, Rongyan Zhou, Lanhui Li and Huiqin Zheng

September 2011

High efficiency regeneration and genetic stability analysis of somatic clones of Gynura bicolor DC.

  Gynura bicolor DC. is a perennial vegetable and medicinal plant. It is an important source of anthocyanins. The effects of different growth regulators on callus induction and plant regeneration were evaluated. The best SFC index (8.6) of plant regeneration was obtained in combination of 2,4-D at 2.0 mg/l and BA at 0.5 mg/l, and the frequency of regenerating explants was 78.3%. The...

Author(s): Li-Sha Liu, Ren Li, Yongqin Zhao, Chang-Long Wen, Shuxin Ren and Yang-Dong Guo

September 2011

A comparative study of ATPase subunit 9 (Atp9) gene between cytoplasmic male sterile line and its maintainer line in soybeans

  ATPase subunit 9 gene (Atp9) is an important functional gene in mitochondria, and is closely related with energy supply. RNA editing of atp9 gene was associated with male sterility in plants. In this study, the atp9 gene in soybeans was cloned from asoybean cytoplasmic male sterile line NJCMS2A and its maintainer line NJCMS2B.Sequence alignment was...

Author(s): Wei Jiang, Shouping Yang, Deyue Yu and Junyi Gai

September 2011

Involvement of rootstocks and their hydraulic conductance in the drought resistance of grafted rubber trees

  Improving drought resistance of rubber trees has become a pressing issue with the extension of rubber plantations and the prevalence of seasonal drought. Root system is vital to water and nutrients uptake of all plants, therefore, rootstocks could play decisive roles in drought resistance of grafted rubber trees on a specific scion clone. To investigate the responses of different clone rootstocks and their...

Author(s): An Feng,, Kong Lingxue, Gong Lidan, Wang Zhenhui, and Lin Weifu,

September 2011

Physiological response of selected eragrostis species to water-deficit stress

  Plant water-deficit stress is considered to be one of the greatest threats to world agriculture. In an effort to identify a better performing crop variety of Eragrostis tefunder this stress, the responses of two varieties, white (W) and brown (B) seeded, were compared with those of the resurrection grass Eragrostis nindensis during a dehydration/rehydration cycle. After 6 days of dehydration,...

Author(s): Z. G. Ginbot and Jill M. Farrant

September 2011

Effects of salinity stress on water uptake, germination and early seedling growth of perennial ryegrass

  This research was conducted for the determination of effects of salinity stress on water uptake of seed, germination and early seedling development of perennial ryegrass (Lolium perenne L. cv. Ovation) in the seed laboratory of Field Crops, Department of Agricultural Faculty of Namik Kemal University, Tekirdag, Turkey, 2009. In this study, eight different sodium chloride (NaCl) levels, including...

Author(s): Ilker Nizam

September 2011

Mycorrhizal association of some agroforestry tree species in two social forestry nurseries

  Mycorrhizal colonization of different agroforestry tree species in two social forestry nurseries was investigated. Percentage of Arbuscular mycorrhizal (AM) infection, number of resting spores and AM fungi species varies both in tree species as well as in two different nurseries. This variation is attributed to various factors such as mycorrhizal status and other management practices. In both nurseries,...

Author(s): Pavan Kumar Pindi

September 2011

Edible macrofungi of Edremit Gulf (Balıkesir) in Turkey

  Gulf of Edremit is an important center of trade, coupled with its sandy beaches, shores and towns that are surrounded by green olive trees (Ayvalık, Edremit, Burhaniye and Havran). It is also one of the largest districts of Balıkesir Province. Macrofungi are ideal food for people because they have a fairly high content of protein which contains all of the essential amino acids. They are also a source of...

Author(s): Rıdvan Polat and Selami Selvi

September 2011

Comparison of volatile components of flower, leaf, peel and juice of ‘Page’ mandarin [(Citrus reticulata var ‘Dancy’ × Citrus paradisi var ‘Duncan’) × Citrus clementina]

  The volatile flavor components of flower, leaf, peel and juice of ‘Page’ mandarin were investigated in this study. Flower components were extracted by using ultrasound (US) water bath apparatus and then eluted by n-pentane : diethylether (1:2) solvent. Leaf flavor components were extracted by using water distillation method and then eluted by using n-hexane solvent. Juice flavor components were...

Author(s): Behzad Babazadeh Darjazi

September 2011

Antimicrobial activities of the bacteriocin-like substances produced by lactic acid bacteria isolated from Moroccan dromedary milk

  A total of 450 different colonies, isolated from 25 samples of dromedary milk collected from Laâyoune region of Morocco, were tested for antimicrobial compounds production. Out of these, 30 were determined to be lactic acid bacteria (LAB) and able to inhibit the growth of the indicator strain Listeria innocua CECT4030. Seven isolates were selected by the large and clear zones of...

Author(s):   El ouardy Khay, Mohamed Idaomar, Lorenzo Miguel Pastrana Castro, Paula Fajardo Bernárdez, Nadia Skali Senhaji and Jamal Abrini

September 2011

Isolation and characterization of Pseudomonas putida WLY for reactive brilliant red x-3b decolorization

  Using the BMM medium containing 100 mg/L of reactive brilliant red X-3B, a decolorizing bacterium with higher decolorization activity was isolated and it showed a decolorization zone of 10 mm; this decolorizing bacterium was identified as Pseudomonas putida WLY based on physiological and biochemical characteristics as well as 16srRNA sequence. In this study, the effects of P....

Author(s): Guanghua Yang, Yucai He, Zhiqiang Cai, Xiyue Zhao, Liqun Wang and Li Wang

September 2011

Growth and physiological responses to water and nutrient stress in oil palm

  The research was conducted to detect changes in growth, physiology and nutrient concentration in response to two watering regimes (well-watered and water-stress conditions) and to two nutrient regimes (with or without fertilization) of oil palm. Under stress conditions, changes in plant growth, dry matter allocation, relative water content, leaf relative conductivity, leaf N, P and K concentration are usually...

Author(s): Cheng-xu Sun, Hong-xing Cao, Hong-bo Shao, Xin-tao Lei and Yong Xiao

September 2011

Total phenolic, condensed tannin and antioxidant activity of four Carya species from China

  Different species of functional agricultural crops may vary in antioxidant capacities. In this study, the antioxidant activities of methanol extracts from four species ofCarya genus were compared by various antioxidant assays, including the reducing power, 1,1-diphenyl-2-pycrylhydrazyl (DPPH) radical scavenging activity and the superoxide anion scavenging activity. The reducing power of extracts...

Author(s): Zhiping He, Maorun Fu and Linchun Mao

September 2011

Effect of cooking methods on chemical composition, quality and cook loss of camel muscle (Longissimus dorsi) in comparison with veal

  Camels are resistant to severe and dry weather conditions and their potential for meat production in such a situation are unique. However, despite the ability of camel meat to supply nutritional deficiencies (such as iron deficiency), it seems that replacement of veal with camel meat can be cheaper source for providing protein, minerals and energy. On the other hand, excessive consumption of fat and...

Author(s): Parang Nikmaram, Mohamad Said Yarmand and Zahra Emamjomeh

September 2011

Comparison of yolk fatty acid content, blood and egg cholesterol of hens fed diets containing palm olein oil and kilka fish oil

  The purpose of this study was to compare the effects of dietary palm olein oil (POO) and Kilka fish oil (KFO) on yolk fatty acid content, ratio of fatty acids (FAs), antibody titre, and blood and yolk cholesterol of laying hens. One hundred White Hy-Line 26-wk-old (W-36) hens were allotted to 6 dietary treatments containing 0, 1.5, 3 and 4.5% POO or 2 and 4% KFO. The FAs and cholesterol content of...

Author(s): S. J. Hosseini-Vashan, N. Afzali, A. Golian, A. Allahressani, M. A. Nasseriand M. Mallakeneh

September 2011

Increasing gland number and red pigments in St. John’s wort in vitro culture: Influence of mannitol, sucrose and hydrolyzed casein

  In order to develop a protocol for increasing the gland number and red pigments ofHypericum perforatum, this study was carried out to evaluate the effect of hydrolyzed casein (0.0 and 500 mg l-1), mannitol (0.0, 5 and10 g l-1) and sucrose (20 and 30 g l-1) on the synthesis of these pigments and glands on the produced leaves. Leaf discs of in vitro plantlets, were prepared and cultured on MS medium...

Author(s): Samaneh Kazemiani and Alireza Motallebi-Azar

September 2011

Effect of 6-benzylaminopurine (BAP) in different basal media on shoot multiplication of Aquilaria hirta and detection of essential oils in the in vitro shoots

  Aquilaria hirta, locally known as ‘chandan’ or ‘chandan bulu’, is used interchangeably with Aquilaria malaccensis (karas) to produce gaharu. With the decline in the A. malaccensis populations, harvesting of this precious wood has begun to extend to A. hirta. There is also an increasing interest to plant karas trees and related species. Therefore, a rapid...

Author(s): Nor Hasnida Hassan, Nor Azah Mohd Ali, Fadhilah Zainudin and Haliza Ismail

September 2011

Study on the serum oxidative stress status in silicosis patients

  To determine whether oxidative-stress damage play an important role in the mechanism of silicosis, the oxidative stress parameters were investigated in silicosis patients and controls group. 128 silicosis patients and 130 healthy controls were included. The serum superoxide dismutase (SOD) activity and the levels of malonyldialdehyde (MDA) and glutathione (GSH) were analyzed. The levels of GSH and MDA in...

Author(s): Jun-Ling He, Juan-Wen Zhang, Guo-Cai Lv, Ying Zhao, Xiao-Ping Hong

September 2011

Direct multiple shoot induction and plant regeneration from dormant buds of Codonopsis pilosula (Franch.) Nannf.

  An efficient and reproducible protocol for in vitro plant multiplication system via direct organogenesis from dormant buds of Codonopsis pilosula Nannf was developed. Multiple shoots were induced at a frequency of 75% after nine weeks on Murashige and Skoog (MS) medium supplemented with BAP (1.0 mg/l), NAA (0.5 mg/l), 3% sucrose and 0.7% agar. Approximately, 15 to 18 shoots were...

Author(s): Y. H. Zhang, S. F. Gao, T. Du, H. G. Chen, H.Z.Wang, T.T.Zhu and J. W. Zhang

September 2011

Comparison of apical sealing ability of resected mineral trioxide aggregate, gutta-percha and a resin-based root canal filling material (resilon)

  In the case of limited access in endodontic surgery, an alternative approach includes obturation of the canal with mineral trioxide aggregate (MTA) prior to surgery. Following the setting of MTA, endodontic surgery is carried out by resecting the root-end and exposing the set MTA without cavity preparation. This may also be performed with other retrofilling materials. This study was designed to...

Author(s): Mohammad Froughreyhani, Amin Salem Milani, Saeed Rahimi, Sahar Shakouie and Somaieh Fateh

September 2011

Mutagenic and antimutagenic potentials of fruit juices of five medicinal plants in Allium cepa L.: Possible influence of DPPH free radical scavengers

  Mutagenic and antimutagenic activities of freeze dried fruit juices (FDFJ) of Morinda elliptica Ridl. (Rubiaceae), Morinda citrifolia L. (Rubiaceae), Averrhoa bilimbi L. (Oxalidaceae), Phyllantus acidus (L.) Skeels (Phyllantaceae) and Myristica fragrans Houtt. (Myristicaceae) in Allium cepa L were evaluated. Testing the mutagenic activity, onions...

Author(s): Akinboro Akeem, Kamaruzaman Bin Mohamed, Mohd Zaini Asmawi and Othman Ahmad Sofiman

September 2011

Genetic and phylogenetic analysis of ten Gobiidae species in China based on amplified fragment length polymorphism (AFLP) analysis

  To study the genetic and phylogenetic relationship of gobioid fishes in China, the representatives of 10 gobioid fishes from 2 subfamilies in China were examined by amplified fragment length polymorphism (AFLP) analysis. We established 220 AFLP bands for 45 individuals from the 10 species, and the percentage of polymorphic bands was 100%. The percentage of polymorphic loci within species ranged from 3.61 to...

Author(s): Gang Han,#, Na Song#, Li yan Zhang, Xi fu Sun and Tian xiang Gao

September 2011

A novel expression cassette for the efficient visual selection of transformed tissues in florist's chrysanthemum (Chrysanthemum morifolium Ramat.)

  Constructs carrying visual reporter genes coupled with efficient promoters could facilitate the process of identification and selection of stable transformants in recalcitrant crops. Here, a novel construct utilizing a ribulose-1,5-bisphosphate carboxylase (RbcS) promoter combined with the green fluorescent protein (GFP)reporter gene to initiate very high expression of GFP in florist's...

Author(s): Jing Mao,Geert Stoopen, Maarten A.Jongsma and Cai-yun Wang

September 2011

Genetic diversity of Escherichia coli isolated from commercial swine farms revealed by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and repetitive extragenic palindrome PCR (REP-PCR)

  The objective of this study was to use enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and repetitive extragenic palindrome PCR (REP-PCR) for the analysis of genetic diversity among Escherichia coli strains isolated from commercial swine farms in Sichuan province of China. Thirty four strains of E. coli were selected by selective medium and conventional biochemical...

Author(s): Li Wan, Zhi-Biao Wang, Qi-gui Yan, Xu Wang, Yan Lei, Lan Zuo, Yu Cheng, Yu-Peng Ren and Wan-zhu Guo

September 2011

Optimization of nutritional requirements and ammonium feeding strategies for improving vitamin B12 production by Pseudomonas denitrificans

  Statistical experiment design and data analysis were used to establish the major factors in a chemically defined medium and to develop an ammonium control strategy to optimize the specific vitamin B12 production rate (Yp) ofPseudomonas denitrificans. Through Plackett-Burman design, the major factors of glucose, ammonium sulfate and KCl were selected as the significant factors...

Author(s): Ze-Jian Wang, Ping Wang, Ju Chu and Si-Liang Zhang

September 2011

The immunological response of RB51 vaccinated buffalo calves using brucella periplasmic proteins as ELISA antigen

  Immune status of RB51 vaccinated buffaloes was evaluated using tube agglutination test (TAT) and ELISA, using both periplasmic protein antigen (PPA) and lipopolysaccharide antigen (LPS). For this purpose, three groups of buffalo calves were used. The first one received S19 vaccine subcutaneously; the second was vaccinated once subcutaneously with RB51 vaccine. The third (control) group was injected similarly...

Author(s): Khaled A. Abd El-Razik, Salama M. Abd El-Hafez, Waleed S. Shell and Yasser A. Ghazi