African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12182

Table of Content: 8 February 2010; 9(6)

February 2010

No more recalcitrant: Chickpea regeneration and genetic transformation

  Chickpea production is limited worldwide because of abiotic and biotic stresses. Efforts to overcome these production constraints through traditional breeding are difficult due to limited genetic variation. Novel regeneration is pre-requisite for genetic transformation offers the opportunity to overcome hybridization barriers and introduce novel genes for resistance. Although direct gene transfer via direct...

Author(s): Firoz Anwar, P. Sharmila and P. Pardha Saradhi

February 2010

Optimization of RAPD-PCR reaction system for genetic relationships analysis of 15 camellia cultivars

  With orthogonal analysis by L27(36), the random amplified polymorphic DNA (RAPD)-PCR optimization reaction system for camellia were obtained. Results showed that the optimization system was 10×PCR Buffer (2.5 μL), 25 mM MgCl2(2.5 μL), 2.5 mM dNTPs (2.0 μL), 20 μM primer (1.0 μL), Tag (1.5 U), temple DNA (40 ng or so) and added ddH2O to the total volume 25 uL; suitable annealing...

Author(s): X. F. Wang, W. H. Zheng, H. X. Zheng, Q. Q. Xie, H. Y. Zheng, H. Tangand Y. L. Tao

February 2010

Association of single nucleotide polymorphisms in IGF-I, IGF-II and IGFBP-II with production traits in breeder hens of Mazandaran native fowls breeding station

  The purpose of this study was to detect the polymorphism in IGF-I, IGF-II and IGFBP-II marker loci and their association with body weight at 8 weeks, average egg weight and total number of eggs laid during first 12 weeks after flocks maturity in breeder hens of native fowls. Blood samples were collected randomly from 160 individuals and genomic DNA was extracted using modified salting out method. A set of...

Author(s): Alireza Khadem, Hassan Hafezian and Ghodrat Rahimi-Mianji

February 2010

DNA sequence and prokaryotic expression analysis of vitellogenin from Antheraea pernyi

  In this study, the DNA sequence of vitellogenin from Antheraea pernyi (Ap-Vg) was identified and its functional domain (30-740 aa, Ap-Vg-1) was expressed inEscherichia coli BL21 (DE3) cells. The recombinant Ap-Vg-1 proteins were purified and used for antibody preparation. The results showed that the intact DNA sequence of Ap-Vg consisted of 8124 bp including 6 exons (2258, 205,...

Author(s): Baojian Zhu, Lei Wang, Chaoliang Liu and Zenta Kajiura

February 2010

Molecular cloning and characterization of a novel expressed sequence tag (EST) associated with fecundity in goats

  To screen the genes controlling the fecundity traits in goats, a DDRT-PCR technique was applied. We found a new EST which highly expressed in Chinese native prolific goat breed, Haimen goats. There exists a difference of EST expression level between the prolific and non-prolific goat breed, indicating EST might associate to fecundity in goats. A full-length cDNA with 2253 base pairs was obtained by the...

Author(s):   Y. Q. He, J. Kong, X. K. Ma, C. X. Zhang, X. Y. Liu and H. Q. Chen  

February 2010

The effects of drought stress on the activity of acid phosphatase and its protective enzymes in pigweed leaves

  A model of drought was created on pigweed and the effects of drought stress on the activity of acid phosphatase and its protective enzymes were examined. The pot-cultured pigweeds were divided into 4 groups (ten plants per group) when they reached 6 leaves. (1) In the control group, the culture media contained 70 - 85% of field moisture capacity, (2) In the second group, the mild drought stress group, the...

Author(s): Sun Cunhua, Du Wei, Cheng Xiangling, Xu Xinna, Zhang Yahong, Sun Dong and Shi Jianjie

February 2010

Investigations on pests, diseases and present early warning system of apple orchards in Isparta, Turkey

  As a result of three year surveys performed in the apple orchards in Isparta region, 19 pest species belonging to 4 orders were determined and it was found that the main pest was codling moth. Most of the predators and parasitoids were effective against aphids and they were mostly found in the orchards where selective pesticides were used. Apple scab and powdery mildew were the most common and important...

Author(s): Gürsel Karaca, Ismail Karaca, Nejla Yardimci, Ozan Demirözer, Baran Aslan and Handan Çulal Kiliç

February 2010

Effects of salinity on sucrose metabolism during tomato fruit development

  The objective of this study was to investigate the effects of salt stress on content of carbohydrate and activity of sucrose-metabolizing enzyme and gene expression patterns and to provide a new evidence for tolerating salt stress of cultivated tomato. The related enzymes’ activities of sucrose metabolism including invertase,sucrose synthase and sucrose phosphate synthase and the expression of acid...

Author(s): Shaowei Lu, Tianlai Li and Jing Jiang

February 2010

In vitro shoot multiplication of Ziziphus spina-christi by shoot tip culture

  In vitro shoot multiplications were obtained successfully from shoot tips of Ziziphus spina-christi by placing explants into solidified medium (MS medium) supplemented with 0.01 mg/l NAA and 0.1 mg/l BA or 0.1 mg/l IAA and 1.0 mg/l kinetin. It was concluded that lower concentrations of all cytokinin studied were better for lateral bud proliferation and that BA and 2IP were better than...

Author(s):   Mohammed Abdulaziz Al-Sulaiman and Mohammed Najeb Barakat  

February 2010

Nanobiotechnology for the production of biofuels from spent tea

  Bioenergy is the only alternative and cheap source of energy which can be made easily available to the world. The present experiment included three steps for the conversion of spent tea (Camellia sinensis) into biofuels. In the first step, spent tea was gasified using Co nano catalyst at 300°C and atmospheric pressure. Catalytic gasification of spent tea yielded 60% liquid extract, 28% fuel gases and 12%...

Author(s): Tariq Mahmood and Syed Tajammul Hussain

February 2010

A study on nitrogen removal efficiency of Pseudomonas stutzeri strains isolated from an anaerobic/anoxic/oxic wastewater treatment process

  In order to improve the nitrogen removal efficiency in an anaerobic/anoxic/oxic treatment plant, a strain with high nitrification and denitrification capability was isolated from a specific anaerobic/anoxic/oxic treatment process. The characteristics of isolate were experimentally analyzed. By using the nitrogen balance method, the total nitrogen loss was calculated to be 40.1% (w/w) when...

Author(s): Yi Wen, Yuan Ren, Chao-Hai Wei, Kai-Yuan Li, Fang-Min Lin and Xue-Yong Chen

February 2010

Use of Aspergillus wentii for biosorption of methylene blue from aqueous solution

  In this study, Aspergillus wentii was used as a biosorbent for the adsorption of methylene blue from aqueous solution. The effects of contact time, initial dye concentration, solution pH and temperature on biosorption were investigated. The contact time required (that is, the equilibrium time) for maximum dye biosorption was found to be 120 min. The amount of the dye biosorbed increased with...

Author(s):   Bilal Acemioğlu, Metin Kertmen, Metin Diğrak, M. Hakkı Alma  

February 2010

Optimization of lipase production by Staphylococcus sp. Lp12

  A bacterial strain isolated from an oil contaminated soil, identified asStaphylococcus sp. Lp12 was screened for lipase activity on tributyrin agar and spirit blue agar medium. Maximum lipase production was observed at 48 h of growth (3.5 Eu/ml). Peptone was found to be as an ideal nitrogen source for production at a concentration of 1.0% (4.25 Eu/ml). Addition of any nitrogen source other than peptone...

Author(s): Pallavi Pogaku, A. Suresh, P. Srinivas and S. Ram Reddy

February 2010

Comparative study of the chemical composition of the essential oils from organs of Annona senegalensis Pers. oulotricha le Thomas subspecies (Annonaceae)

  The chemical composition of the essential oils from leaves, stems bark, roots bark, epicarp and mesocarp of Annona senegalensis Pers., oulotricha Le Thomas subspecies (Annonaceae), growing in Brazzaville (Congo), were analyzed by CG and CG-MS. These oils essentially contain sesquiterpenic compounds (58.3 - 97.7%), dominated by oxygenated sesquiterpenes (21.8 - 88.3%), with elemol (13.2 -...

Author(s): Nkounkou-Loumpangou Célestine, Gouollaly Tsiba, Mahmout Yaya, Elouma Ndinga Arnold Murphy, Ouamba Jean-Maurille, Chalchat Jean Claude and Figuérédo Gilles

February 2010

Study on serum fluorescence spectra based on wavelet transform

  Blood plays an important role in clinical diagnosis and treatment and as such, the analysis of blood spectrum will be of very important practical significance. Serum fluorescence emission intensity is closely related with the excitation wavelength; when the excitation wavelength is 230 nm, the blood lipid concentration and fluorescence intensity was significantly correlated. On the contrary, blood sugar was...

Author(s): Zhu Weihua, Zhao Zhimin, Guo Xin and Hong Xiaoqin  

February 2010

High performance liquid chromatographic determination of proguanil after derivatisation with sodium benzoxazole-2-sulphonate

  A simple, fast and reproducible method for the determination of proguanil using high performance liquid chromatographic with UV/Fluorescence detection is described. Proguanil was derivatised to its corresponding derivative [(N1-(4-chlorophenyl)-N5-(1 -methyl ethyl) imidocarbonimideamide-N-benzoxazole]. The derivatisation reaction was conducted in methanol at 60°C using sodium benzoxazole-2-sulphonate...

Author(s): G. O. Adewuyi, O. Olubomehin and A. W. Ayanniyi

February 2010

Effects of road transportation on excitability scores of pigs administered with ascorbic acid during the hot-dry season

  This study was carried out in order to determine the effect of eight-hour road transportation on the excitability scores of pigs administered ascorbic acid (AA) during the hot-dry season in Northern Nigeria. Thirteen experimental pigs were administered with AA orally at 100 mg/kg, while ten control pigs were given only distilled water orally. Excitability score of each pig was determined 30 min before and...

Author(s): Olayinka Oluwafemi Asala, Joseph Olusegun Ayo, Adeshina Yahaya Adenkola and Ndazo Salka Minka

February 2010

The inhibitory effect of Curcuma longa extract on telomerase activity in A549 lung cancer cell line

  Telomerase is reactivated in lung cancer cells, the most prevalent cancer worldwide, but not normal cells. Therefore, targeting it, preferably with natural compounds derive from medicinal plant such as curcumin, could have important effect on treatment of lung cancer. Curcumin, derived from Curcuma longarhizome, has many anti-cancer activities. Therefore, the main objective of current work was to study...

Author(s): Pourhassan Mohammad, Zarghami Nosratollah, Rahmati Mohammad, Alibakhshi Abbas and Ranjbari Javad

February 2010

The effect of phytosphingosine isolated from Asterina pectinifera on cell damage induced by mite antigen in HaCaT cell and antibacterial activity against Staphylococcus aureus

  The current study was to investigate the positive protective effects of phytosphingosine (PS) against mite antigen and Staphylococcus aureus, etiological causes of an atopic dermatitis. To achieve this aim, PS was isolated from starfish,Asterina pectinifera, using high-performance liquid chromatography and was elucidated with nuclear magnetic resonance spectrometry. In the present experiment, PS,...

Author(s): Gui Hyang Choi, Bo Ae Kim, Chan Ik Park and You Young Kim