August 2012
Cloning and expression analysis of dihydroxyflavonol 4-reductase (DFR) in Ascocenda spp.
Dihydroflavonol 4-reductase (DFR) gene is a key gene of anthocyanins biosynthesis pathway, which represent an importance pathway for orchid flower. In this study, cloning and expression analysis of DFR gene in Ascocenda spp. were carried out. Nucleotide analysis revealed that the Ascocenda DFR gene was 1,056 bp in length, and encoded a protein of 351 amino acid...
August 2012
Crude extract of Nigella sativa inhibits proliferation and induces apoptosis in human cervical carcinoma HeLa cells
Cervical cancer is a major cause of morbidity in women worldwide and chemotherapy for this cancer seems unsatisfactory, which demands exploring new therapeutic options. The seeds and oil from Nigella sativa have been reputed to have many curative properties in traditional medicine. Utilizing different techniques (4',6-diamidino-2-phenylindole (DAPI) staining, deoxyribonucleic acid (DNA)...
August 2012
Identification and characterization of longevity assurance gene related to stress resistance in Brassica
Brassica is a very important vegetable group worldwide and different stresses are a major concern for these crops. Enhancement of resistance against biotic and abiotic stresses by exploiting stress resistance related genes offers the most efficient approach to address this concern. In this study, a stress resistance related gene was identified from the full-length cDNA library of Brassica...
August 2012
Studies on effects of salicylic acid and thiourea on biochemical activities and yield production in wheat (Triticum aestivum var. Gimaza 9) plants grown under drought stress
Drought is a problem of water deficit in the soil. It causes restrictions of wheat plants growth and productivity. In the present approach, we explored new methods for drought managment. Wheat grains were presoaked in ~1 mmol salicylic acid (SA). Wheat was left to grow with 40% soil water holding capacity in pots until yielding stages. The leaves which were produced were two-times sprayed with 2.5 and 5 mmol...
August 2012
Improved techniques to enhance the yield of paddy straw mushroom (Volvariella volvacea) for commercial cultivation
Cultivation of Volvariella volvacea in circular compact bed method recorded maximum yield of 972.8 g/bed (biological efficiency of 23.8%). Seeding the beds with 15 and 20 days old spawn with well developed chlamydospores contributed for significantly higher yields. Oil palm bunch waste in combination with rice straw or alone recorded significantly higher yields of 876.3 and 832 g/bed, and cotton...
August 2012
Growth, chemical components and ensiling characteristics of king grass at different cuttings
In order to effectively use and ensile king grass (Pennisetum purpureum ×Pennisetum americanum), the present research investigated growth rate, yield, chemical components and silage fermentation quality of different cuttings. King grass was harvested four times, and the 1st and 3rd cuttings were ensiled directly or after wilting for 12 and 24 h. The results showed that the dry matter...
August 2012
Application of chitosan padded rice and wheat husk for the removal of reactive dye from aqueous solution
Industrial waste especially textile effluents contain many harmful ingredients, which cause environmental problems. In the present study, the removal of reactive red 195 dye was carried out using chitosan padded wheat husk (CWH) and rice husk (CRH) adsorbents. Chitosan was prepared in the laboratory from chitin which was obtained from crab shells by adopting reported method. The removal of dye was carried out...
August 2012
The isolation and characterization of endophytic microorganisms from Hyptis marrubioides Epling roots
Endophytic microorganisms asymptomatically colonize healthy plant tissues and may be related to the plant's resistance to attack by pathogens or even to the synthesis of secondary metabolites. The present study was aimed at isolating and characterizing endophytic strains from the root system of Hyptis marrubioides. Coarse and fine root fragments were collected for diaphanization and surface...
August 2012
The effect of smoking on changes in functional attributes of Mozzarella cheese
Smoking of cheeses significantly modifies not only sensory attributes, but also determines the form of their further utilisation, packaging method and shelf life. The aim of thIS study was to evaluate functional characteristics of smoked and unsmoked Mozzarella cheeses subjected to 4-week storage. It was found that smoked cheese contained 7% less water than unsmoked cheese. Meltability of smoked cheese before...
August 2012
A new screening method for selection of desired recombinant plasmids in molecular cloning
One of the problems in cloning process is the low concentration of gene fragment and vector following gel extraction stage which may lead to decreased likelihood of effective ligation. Regarding the facts of this study, after digestion process, the products directly were subjected to ligation. Due to the presence of three antibiotic resistance genes in the reaction, a new strategy based on design of selective...
August 2012
The influence of the different content of protein fractions in sows’ milk in piglet rearing
The aim of this study was to investigate the influence of the percentage content of protein fractions in total protein of sow`s colostrums and milk and their influence on the traits related with piglet rearing. The animal specimens were 20 sows of the native ZÅ‚otnicka White breed. ZÅ‚otnicka pigs were subjected to the National Genetic Resources Conservation Programme. Colostrum and milk were...
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