African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12427

Table of Content: January 2005; 4(1)

January 2005

Restriction mapping of Cucurbitae Pepo L. chloroplast DNA Pstl 9.8 kb fragment and detection of ORF 2280 transcripts

  Using tomato heterologous probe, the ORF 2280 was located on the PstI 9.8 kb fragment in the inverted repeat A (IRA) and on the PstI 12.1 kb fragment in the inverted repeat B (IRB) on the physical map of squash chloroplast DNA.  A fine map of the PstI 9.8 kb fragment was developed using SalI, BamHI and EcoRI restriction enzymes.Two transcripts of 1.4and 2.6 kbs of ORF 2280 of squash plastid DNA were...

Author(s): Silas D. Obukosia, Charles D. Boyer, Christina M. Richards

January 2005

Genetic and nongenetic effects on the number of ovarian follicles and oocyte yield and quality in the bovine local (Oulmes Zaer), exotic breeds and their crosses in Morocco

The effects of genetic and non genetic factors on the number of ovarian follicles and oocyte yield and quality in the bovine local breed (Oulmes Zaer), exotic breeds and their crosses in Morocco was investigated. In this study, females in very bad body conditions (BCS < 2) were not slaughtered and the average. The body condition scores (BCS) was 2.94 ± 0.89. Although some individual values were out of the...

Author(s): A. Fassi Fihri, H. Lakhdissi, L. Derqaoui, Kh. Hajji, M. Naciri  A. Goumari

January 2005

Production and partial characterization of a thermostable amylase from ascomycetes yeast strain isolated from starchy soils

  An amylolytic yeast strain was isolated from starchy soils (flour mills environment, flour market, cassava farms after harvesting and transformation of tubers) and its enzyme productivity and activity evaluated. The enzyme synthesis was optimum at 30°C when initial pH of fermentation medium was 4.5. After extraction and partial purification, the enzyme remained stable in a range of temperature and pH...

Author(s): Bertrand Tatsinkou Fossi, Frederic Tavea, Robert Ndjouenkeu

January 2005

Hydrolysis of raw tuber starches by amylase of Aspergillus niger AM07 isolated from the soil

  Eight Aspergillus niger strains which produced strong starch degrading amylase were isolated from the soil using a medium containing Remazol Brilliant Blue (RBB) starch as substrate. Amylase production was detected by the disappearance of the blue colour around the colony. Among the isolates, A. niger AM07 produced the largest clear zone (7.0mm) on Remazol Brilliant Blue (RBB) agar plate...

Author(s): Omemu A. M. , Akpan, I , Bankole M. O. and Teniola O. D. 

January 2005

Identification of over producer strain of endo-β-1,4-glucanase in Aspergillus Species: Characterization of crude carboxymethyl cellulase

  Cellulases are a group of hydrolytic enzymes capable of degrading cellulose to smaller sugar components like glucose units. These enzymes are produced by fungi and bacteria. The aim of this research was to identify a Aspergillus species with over production of endo-β-1,4-glucanase. Properties of endo-β-1,4-glucanase/carboxymethylcellulase (CMCase) from a culture filtrate of...

Author(s): Habib Onsori , Mohammad Raza Zamani, Mostafa Motallebi, and Nosratollah Zarghami

January 2005

Evaluation of microbial systems for bioremediation of petroleum refinery effluents in Nigeria

  The potential of various organisms to catabolize and metabolize organic compounds has been recognized as potentially effective means of disposing of hazardous wastes. Phenolic compounds has long been recognized as one of the most recalcitrant and persistent substance in petroleum refinery effluents. This is a cause of some concern because of the high toxicity and of this compound. Bioremediation of phenolic...

Author(s): Ojumu T.V., Bello O.O., Sonibare J.A. and Solomon B.O.

January 2005

Taxonomic perspective of plant species yielding vegetable oils used in cosmetics and skin care products

  A search conducted to determine the plants yielding vegetable oils resulted in 78 plant species with potential use in cosmetics and skin care products.  The taxonomic position of these plant species is described with a description of vegetable oils from these plants and their use in cosmetic and skin care products.  These species belonged to 74 genera and 45 plant families and yielded 79 vegetable...

Author(s): Mohammad Atharand Syed Mahmood Nasir

January 2005

Study on transformation of cowpea trypsin inhibitor gene into cauliflower (Brassica oleracea L. var. botrytis)

  Cowpea Trypsin Inhibitor (CpTI) gene was transferred into cauliflower byagrobacterium-mediated transformation method, and 14 transgenic cauliflower plants were obtained. Cotyledons and hypocotyls were used as explants. The putative transformants were assayed by PCR and Southern blotting analysis. The results indicated that CpTI gene was transferred into cauliflower successfully. The result of preliminary...

Author(s): LV Lingling   Lei Jianjun   Song Ming    Li Liyun   Cao Bihao

January 2005

Effect of red and far-red light on inhibition of hypocotyl elongation in ecotypes of Betula pendula Roth

  Plants sense the quality, quantity, and duration of light signals and use them to optimise their growth and development. These signals are perceived by special light receptors of which the phytochrome pigment system is one of the most important for photo­morphogenetic responses. Using special diodes that emit monochromatic light, we studied the effect of red (R), far-red (FR) and R+FR combinations on...

Author(s): Berhanu A. Tsegay, Jorunn E. Olsen, and Olavi Junttila

January 2005

Nodulation and growth response of Sesbania sesban (L.) Merr. to increasing nitrogen (ammonium) supply under glasshouse conditions

  A glasshouse experiment was carried out to study the effect of ammonium-N on the nodulation, growth and N-uptake of Sesbania sesban (L.) Merr. inoculated with an effective Mesorhizobium (S. sesban) strain.  Ammonium-N was supplied twice weekly as 100 ml of nutrient solution at concentrations of 0, 100, 200, 400, 600 and 800 mg N/l.  The seedlings were grown in pots with...

Author(s): S.A. Indieka and D.W. Odee

January 2005

Recovery of herbicide-resistant Azuki bean [Vigna angularis (Wild.), Ohwi & Ohashi] plants via Agrobacterium- mediated transformation

  Transgenic azuki bean [Vigna angularis (Willd.) Ohwi & Ohashi] plants expressing the hygromycin phosphotransferase (hpt), green fluorescent protein (sgfp) and phosphinothricin acetyltransferase (bar) genes were obtained by Agrobacterium-tumefacients - mediated transformation. A total of 210 epicotyl explants were inoculated with A. tumefaciens strain EHA105, harboring the...

Author(s): Mutasim M. Khalafalla, Hany A. El-Shemy, Rahman S. Mizanur, Masayoshi Teraishi, Masayoshi Teraishi, Masao Ishimoto

January 2005

Induction of callus and somatic embryogenesis from cotyledon explants of Parkia biglobosa (Jacq.) Benth

To explore the potential for in vitro rapid regeneration of Parkia biglobosa, an endangered multipurpose woody angiosperm, cotyledon explants obtained from 7-day old aseptically germinated seedlings were cultured on Murashige and Skoog (MS) basal medium supplemented with 0.4-1.0 mg/L naphthalene acetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D). Cotyledon explants were placed either with the...

Author(s): Amoo, Stephen Oluwaseun and  Ayisire, Benjamin Erhinmeyoma

January 2005

Toxicity studies in rats fed nature cure bitters

  Graded doses of Nature Cure Bitters (NCB) were administered daily (100, 200 and 400 mg/kg p.o) to rats for 28 days and the effects on body weight, organ weight, clinical signs, gross pathology, haematology, histology and serum biochemical parameters were evaluated. The relative weights of the heart, liver and testes of treated rats were unaffected in contrast to a significant increase in the relative weights...

Author(s): Stanley O. Aniagu, Florence C. Nwinyi, David D. Akumka, Gloria A. Ajoku, Sunday Dzarma, Kazeem S. Izebe, Matthew Ditse, Patrick E. C. Nwaneri, Charles Wambebe and Karynius Gamaniel

January 2005

The pattern of infection and in vivo response to Chloroquine by uncomplicated Plasmodium falciparum malaria in northwestern Nigeria

  The pattern of infection and in vivo response of uncomplicated Plasmodium falciparummalaria to Chloroquine as first line drug and Quinine, Halofantrine or Sulfadoxine-Pyrimethamine as second line medications was evaluated at nested sentinel points, including Government and Private Practices, for three consecutive months. 559 cases were evaluated of which 22.5% failed on Chloroquine therapy. The...

Author(s): Bello SO, , Muhammad BY, Bello AY, Ukatu AI, Ahmad BM, Adeneye AA,Cherima JY

January 2005

Pitfalls in the routine diagnosis of Staphylococcus aureus

 Two hundred isolates of Presumed Staphylococcus aureus from routine clinical specimens, collected from two government hospitals in Abha, Saudi Arabia, had their identity verified. We used the tube coagulase test as our gold standard. Twenty (10%) of the isolates were mis-identified. Reliance by the two laboratories on DNase test, Mannitol fermentation reaction and improper performance of the coagulase...

Author(s): C.S.S. Bello and A. Qahtani

January 2005

Activity staining method of chitinase on chitin agar plate through polyacrylamide gel electrophoresis

  A method for detection of chitinase activity on chitin agar plate after polyacrylamide gel electrophoresis is described. Different staining dyes such as calcofluor white M2R, fluorescein isothiocyanate, rhodamine B, ruthenium red and congo red were separately incorporated in chitin agar plates. After running polyacrylamide gel electrophoresis, the gel was transferred onto chitin agar plate containing...

Author(s): Vipul Gohel,  Pranav  Vyas and  H.S. Chhatpar

January 2004

Effect of Nauclea latifolia leaves aqueous extracts on blood glucose levels of normal and alloxan-induced diabetic rats

  In Northern Nigeria, different parts of Nauclea latifolia (family: Rubiaceae) are prescribed as  remedy for diabetes mellitus. This study was designed to evaluate the hypoglycemic property of aqueous extract of the leaves of the plant in normal and alloxan-induced diabetic rats. The aqueous extracts at (200 mg/kg bw.) significantly lowered glucose levels (p<0.05) of the diabetic rats by...

Author(s): Gidado A., Ameh D.A. and Atawodi, S.E.

January 2005

Stability of yam starch gels during processing

  To evaluate their aptitude to be used as functional ingredients, twenty one varieties of yam starches of Côte d’Ivoire were submitted to different technological stress such as high temperature treatment, long term freezing and refrigeration, high speed shearing and acidic treatment, in comparison with commercial modified starches. The gel of “kangba” starch (D. Cayenensis-rotundata) is...

Author(s): N’Guessan Georges Amani, Alphonse Kamenan Agnès Rolland-Sabaté, and Paul Colonna

January 2005

Retention of β-carotene in frozen carrots under varying conditions of temperature and time of storage

  Carrots were blanched and stored in a freezer to study the retention of β-carotene. Retention was found to be better in 3 min blanched samples than in 5 min ones. There was an increase in β-carotene content in carrots after 5 days at all storage temperatures: 0, –8, –14, and –18oC. Decrease was found to be insignificant compared to the initial value even after 80 days of storage...

Author(s): Debjani Dutta, Utpal Raychaudhuri, and Runu Chakraborty

January 2005

Export papaya post-harvest protection by fungicides and the problems of the maximal limit of residues

  The post-harvest maturity of papaya fruits is influenced by several environmental  factors  including temperature, light and ventilation of the surrounding wall. The maturity, presence and attacks   or   rots,   and   the accumulation   of   fungicide   residues   in the papaya was evaluated on papaya fruits treated with...

Author(s): Dembele Ardjouma, Traore Sory Karim, Kone Mamadou, and Coulibaly Dotana Ténébé

January 2005

Microbial control of caged population of Zonocerus variegatus using Beauveria bassiana and Metarhizium sp.

  Microbial control of caged populations of Zonocerus variegatus was carried out using indigenous fungal entomopathogens isolated from the grasshopper’s cadaver. Bioassay response indicated a dose-dependent mortality coupled with drastic reduction in food consumption among spores infected grasshoppers. Lethal time (LT50) of 4.6 days for 103 spores/ml and 3.8 days for 107 spores/ml of...

Author(s): O.E. Fagade, S.A. Balogun and C.J. Lomer

January 2005

Histopathology of the gill and liver tissues of the African catfish Clarias gariepinus exposed to lead

  An investigation on the effect of the heavy metal, lead (Pb) on the gill and liver of the African catfish Clarias gariepinus was carried out in the laboratory. One hundred and sixty (160) fingerlings of the fish were exposed to continuous exposure to sub-lethal concentrations (0.006 mg/l and 0.008 mg/l) of lead for a period of three weeks. The liver and gill of fish were removed every 9 days for...

Author(s): Olojo, E. A. A. , Olurin, K. B., mbaka, G. And Oluwemimo, A. D.