African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12393

Table of Content: January 2022; 21(1)

January 2022

Rapid single-tube splice variants typing of the BF gene based on dual-primer RT-PCR amplification that influence resistance/susceptibility to Marek’s disease in chicken

A new effective splice variants typing based on multiplex allele-specific dual-primer RT-PCR assay was developed in a single tube for the rapid detection of the exon 7 splice variant of the BF gene. With 2 pairs of primers, one pair was used for amplifying cDNA fragments containing exon 7 of the BF gene, the other does not contain exon 7 of the BF gene. The templates were amplified in one tube and the type of splice...

Author(s): Yuan-chang Jin, Yu-feng Li, Liang Huang, Jia-jun Zhou, Xue-fang Zhang,  Ran Ma, Meng-lin Lu, Mei-lin Hao, Gang Zeng  and Bo-ping Zeng

January 2022

Molecular and morphological identification of fungi causing canker and dieback diseases on Vangueria infausta (Burch) subsp. rotundata (Robyns) and Berchemia discolor (Klotzsch) Hemsl in lower Eastern Kenya

Drought-tolerant multipurpose fruit trees Vangueria infausta (Burch) subsp. rotundata (Robyns) and Berchemia discolor (Klotzsch) Hemsl are native to Kenya. These fruit tree species are suitable for dryland agroforestry and support local communities with food, medicine, fodder and other necessities. Reports by the local communities indicate that the two species suffer from diebacks and cankers. The aim of this study was...

Author(s): Susan Karani, Jane Njuguna, Steven Runo, Alice Muchugi, Joseph Machua and Phoebe Mwaniki

January 2022

Isolation and characterization of multidrug resistance bacteria from hospital sewage samples, Maharashtra, India

Multiple antibiotic resistance is a major cause of clinical infections worldwide. This study determined the pattern of multidrug bacterial resistance in hospital sewage samples from the Marathwada region of India. Forty-eight isolates of bacteria were obtained from 6 locations of Aurangabad.  An antibiotic sensitivity test was carried out using the disc diffusion method. Among all the antibiotics tested, the...

Author(s): Bhaurao Bapurao Choushette and Rajendra A. Satpute

January 2022

Diversity of Arbuscular mycorrhizal fungi in the three agroecological zones of the Central African Republic

Arbuscular mycorrhizal fungi (AMF) are organisms that can establish symbioses with 80% of terrestrial plants. Soils were collected in three agroecological zones (AEZ) of the Central African Republic (CAR). The aim of this research is to study the diversity of AMF in the CAR. The spore extraction and enumeration, granulometric and physicochemical analyses of each soil were performed. The results showed that AEZ3 has a...

Author(s): Mathurin Djamndo DJASBE,, Hubert Dieu Béni ELIAN, Tahir Abdoulaye DIOP and Denis Omokolo NDOUMOU

January 2022

Identification of potential seed storage protein responsible for bruchid resistance in common bean landraces from Tanzania and Malawi

Bean bruchids are among the most devastating insect pests of common bean that can inflict huge losses in storage. To identify potential resistance to these pests, screening was performed at Sokoine University of Agriculture. Two resistant landraces were identified, viz Kalubungula and KK25. Recombinant inbred (RI) KSy, KSw and ML populations were created from crosses between Soya × Kalubungula, Soworo ×...

Author(s): Caroline Ng’homange Maro, Deogracious Protas Massawe, George Muhamba Tryphone, James Robert Myers, Joel William Davis and Paul Mbogo Kusolwa