African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12300

Table of Content: November 2019; 18(31)

November 2019

Expression profiling, phylogenetic, and structural analyses of a laccase gene from the red palm weevil, Rhynchophorus ferrugineus

Laccases, member of multicopper oxidase (MCO) family enzymes, play crucial roles in insects’ cuticle tanning and pigmentation. The purpose of this study was to identify and characterize a laccase gene from the red palm weevil (RPW), Rhynchophorus ferrugineus. The isolated RPW laccase gene sequence was 3,389 bp, including a 2,163 bp open reading frame that encodes 720 amino acids. The RPW laccase gene conserved the...

Author(s): Babiker M. A. Abdel-Banat and Hamadttu A. F. El-Shafie

November 2019

In vitro regeneration protocol through direct organogenesis for Jatropha curcas L. (Euphorbiaceae) accessions in Ethiopia

Jatropha curcas L. is among important tree crops in the world with a potential for biofuel production. In Ethiopia, there is a soaring investors’ interest to produce Jatropha in the country for biodiesel production. However, insufficient good quality propagation material is a major production constraint. A study was undertaken to establish a protocol for in vitro mass propagation of three Ethiopian Jatropha...

Author(s): Hundessa Fufa, Meseret Tesema and Jiregna Daksa  

November 2019

Effects of genotype and plant growth regulators on callus induction in leaf cultures of Coffea arabica L. F1 hybrid

Coffea arabica, F1 hybrid variety, Ruiru 11 is a highly sought after crop in Kenya due to its alleviated resistance to Coffee Berry Disease and Coffee Leaf Rust coupled with high yield capacity and good cup quality. Access to the variety’s planting materials is limited due to challenges with difficulty in propagation using conventional methods of seed and vegetative propagation; and somatic embryogenesis is...

Author(s): Mwaniki W. I., Lubabali A. H., Asava K. K., Agwanda C. O. and Anami S. E.  

November 2019

Relationship between 2-acetyl-1-pyrroline and aroma in Uganda rice populations with Oryza (barthi, glaberrima and sativa) backgrounds

The sweet popcorn aroma conferred by 2-acetyl-1-pyrroline (2AP) is a highly economic trait of rice grain attracting premium price worldwide. This research study was conducted to determine the levels of 2AP in Ugandan rice lines with the aim of establishing a better understanding on the level and classes of 2AP and aroma phenotype. Concentration of 2AP was assayed using two-dimensional gas chromatography-time-of-flight...

Author(s): David Ocan, Rongrong Zhang, Martin Odoch, Michael Kanaabi,  Angele Pembele Ibanda, Agnes Akwero, Bill Williams Khizzah, Ephraim Nuwamanya, Jimmy Lamo, Melissa Anne Fitzgerald, Venea Dara Daygon and Patrick Rutimbanzigu Rubaihayo

November 2019

Evaluation of aflatoxins levels and molecular identification of toxigenic molds in cereals and cereal-derived breakfast foods in Nigeria

In the last decade, there have been an increase in safety concerns on the aflatoxins contents of cereals and cereal-derived food products in Nigeria. In the current study, evaluation of aflatoxins levels and molecular identification of toxigenic molds recovered from cereals and cereal-derived foods products in Nigeria were studied. Enzyme Link Immunosorbent Assay (ELISA) and high performance liquid chromatography (HPLC)...

Author(s): Michael O. Odo, Fidelis Azi, Ignatius C. Alaka and Veronica N. Nwobasi

November 2019

Whole-genome optical mapping: Improving assembly of Macrophomina phaseolina MS6 through spanning of twelve blunt end chromosomes by obviating all errors and misassembles

Deciphering genetic information through next-generation sequencing (NGS) is considered as the basic platform to unveil in details of an organism. However, as it produces short reads that lead to difficulties in assembly, we generated long scaffold-based optical mapping (OM) data of previously sequenced devastating fungus, Macrophomina phaseolina MS6. In the process, KpnI identified as the most effective restriction...

Author(s): Quazi Md. Mosaddeque Hossen, Md. Shahidul Islam, Emdadul Mannan Emdad, Md. Samiul Haque, Md. Monjurul Alam and Maqsudul Alam,

November 2019

Callus induction in three mosaic disease resistant cassava cultivars in Benin and genetic stability of the induced calli using simple sequence repeat (SSR) and sequence-characterized amplified region (SCAR) markers

The effect of different concentrations of thidiazuron (TDZ), benzyl amino purine (BAP), kinetin and 2,4-dichlorophenoxy acetic acid (2, 4-D) on callus induction in three elite cassava cultivars (agric-rouge, atinwewe and agblehoundo) was evaluated. Leaf explants harvested from greenhouse-grown cassava were sterilised using different concentrations of commercial bleach commonly called Jik (3.85% NaOCl) at different time...

Author(s): Amitchihoué Franck Sessou, Jane W. Kahia, Elijah Ateka, Jerome Anani Houngue, Colombe Dadjo, Peter Njenga and Corneille Ahanhanzo