African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12480

Table of Content: September 2004; 3(9)

September 2004

Optimization of industrial production of rifamycin B by Amycolatopsis mediterranei. IV. Production in the fermentor

Optimization of the physical and physiological parameters of the fermentation process using the gene amplified variant of Amycolatopsis mediterranei (NCH) was carried out. Optimization of the physical parameters by controlling the pH at 6.5 for 3 days then at 7 thereafter and by adjustment of aeration at 1 vvm for 3 days then controlling the dissolved oxygen (DO) at 30% saturation  increased the...

Author(s): El-Tayeb, O.M., Hussein, M. M. M., Salama, A.A. and El-Sedawy, H.F.

September 2004

Antibacterial activity of some actinomycetes isolated from farming soils of Turkey

A total of 50 different actinomycete strains were recovered from farming soil samples collected from Manisa Province and its surrounding. These were then assessed for their antibacterial activity against four phytopathogenic and six pathogenic bacteria. Results indicated that 34% of all isolates are active against, at least, one of the test organisms;Agrobacterium tumefaciens, Erwinia...

Author(s): Mustafa Oskay, A. Üsame Tamer and Cem Azeri

September 2004

Saccharification of banana agro-waste by cellulolytic enzymes

Banana is major cash crop of this region generating vast agricultural waste after harvest. The agro-waste including dried leaves and psuedostem after harvest was used as substrate for the release of sugars. Saccharification of banana agro waste by cellulases of Trichoderma lignorum was investigated. The steam treated agro-waste yielded 1.34 mg/ml of reducing sugars after 24 hr. The size of substrate affected...

Author(s): M.M.V. Baig, M.L.B. Baig, M.I.A. Baig and Majeda Yasmeen

September 2004

Effect of roasting on the aflatoxin contents of Nigerian peanut seeds

Peanut seeds were prepared with variations in roasting conditions. Positive correlations were obtained between loss of aflatoxins in the products and the roasting conditions. Seeds dry- roasted at 140OC for 40 min resulted in 58.8% and 64.5% reductions in AFB1 and AFG1, those roasted at 1500C for 25 minutes resulted in 68.5% and 73.3% reductions in AFB1 and AFG1, respectively. Roasting at150OC for...

Author(s): Ogunsanwo, B.M., Faboya, O.O.P., Idowu O.R., Lawal O.S. and Bankole S.A.

September 2004

Temperature profiles of Agaricus bisporus in composting stages and effects of different composts formulas and casing materials on yield

Three compost formulas using different activator materials were prepared for Agaricus bisporus cultivation. A locally available casing material known as peat of Bolu district and its different combinations with perlite were used. Temperature profiles of all mixtures during composting were measured at every composting stages at various depth in order to determine the compostability level of substrates. Compost...

Author(s): Mehmet COLAK

September 2004

Influence of sex, source, health status and acclimation on the haematology of Clarias gariepinus (Burch,1822)

The influence of sex, source (pond and wild) acclimation and health status on some blood parameters of C. gariepinus was studied.  There were no significant differences between the blood parameters  (haemoglobin (Ht), packed cell volume (PCV), erythrocyte sedimentation rate (ESR), red blood cells (RBC), RBC indices ( MCHC;, MCH: and MCV), white blood cells (WBC) and differential counts (neutrophils,...

Author(s): Gabriel, U.U., Ezeri, G.N.O. and Opabunmi, O.O.

September 2004

Preliminary studies of inhibitions in Aspergillus flavus with extracts of two lichens and Bentex-T fungicide

One ml aqueous concentrations at 0.1, 0.25, 0.5, 1.0, and 5.0 mg/ml of laboratory extracts of Hypogymnia physodes and Ramalina farinacea (Lichens), and Bentex-T, were incorporated separately in a basal broth culture medium inside conical flasks. Mycelial dry weight of Aspergillus flavus, grown on the medium with the extracts of the lichens, was inhibited between 70% - 80% compared to...

Author(s): Suberu H.

September 2004

Microbial contaminants of cultured Hibiscus cannabinus and Telfaria occidentalis tissues

Nine microbial contaminants comprising of five bacteria and four fungi species were isolated from Hibiscus cannabinus  and Telfaria occidentalis cultured tissues.  The rate of occurrence of bacteria isolates was higher than that of fungi. The bacterial isolates includes Pseudomonas syringae pv phaseolicoli,  Bacillus licheniformis,Bacillus...

Author(s): Odutayo O. I. , Oso R. T., Akinyemi B. O. and Amusa N. A.

September 2004

Prevalence of blaSHV genes in clinical isolates of Klebsiella pneumoniae at Saint Camille medical Center in Ouagadougou. Isolation of blaSHV11-like gene

Five bacterial strains (4 Klebsiella pneumoniae and 1 Escherichia coli) representative of pathogenic species and resistant to b-lactam antibiotics are investigated to isolate the genes responsible of b-lactamase activity. The use of engineering techniques enables us to show the widespread of blaSHV genes particularly in clinical isolates of K. pneumoniae. Our results highlighted...

Author(s): Boukaré Zeba Pere Jacques Simporé, Odile G. Nacoulma, Jean-Marie Frere

September 2004

In vitro anti trypanosomal activity of some medicinal plants used in the treatment of trypanosomosis in Northern Nigeria

The in vitro trypanocidal activity of 13 medicinal plants used by local herdsmen in Northern Nigeria for the treatment of trypanosomosis was investigated. Forty-four different extracts prepared from the 13 plants were screened for in vitro activity againstTrypanosoma   brucei brucei. Four of the extracts showed activity against the parasite at minimum concentration of 8.3 mg/ml of...

Author(s): Wurochekke A.U. and   Nok A.J.

September 2004

Assessment of a fruit extract (Sechium edule) on the labeling of blood elements with technetium-99m

Natural products have been widely used by human beings. However, sometimes the biological effects of these products are not fully known. Chayotte (Sechium edule) is a vegetable used in the folk medicine. Red blood cells (RBC) labeled with technetium-99m (99mTc) have several clinical applications. The aim of this work was to evaluate the influence of an extract of chayotte on the labeling of blood elements with 99mTc...

Author(s): Gláucio Diré Feliciano, Maria Luísa Gomes,  Elaine Alves Correia Lima, Roberto Levi Jales,  Mauro Castro Faria and Mário Bernardo Filho