Abstract
Aim: To investigate the suitability of Hibiscus extract mordanted with ferric chloride and counterstained with Van Gieson in the demonstration of brain histomorphology.
Method: 10% formol saline fixed brain tissues from cerebellum, cerebrum and basal ganglia were processed by the paraffin wax technique, sectioned and stained with H&E labeled A, Hibiscus/Van Gieson stain labeled B and Weigert’s Van Gieson stain labeled C as control.
Results: Photomicrographs from all the staining techniques presented all the brain cells especially the molecular cell layer and the granular cell layer of the cerebellum in comparable manner. The cells were stained blue-black with the Hibiscus-Van Gieson (B) and Haematoxylin van Gieson (C) methods.
Conclusion: The suitability of Hibiscus extract-Iron-van Gieson in the histomorphological demonstration of brain tissue is established.
Key words: Hibiscus stain, Van Gieson, neuro-histology, roselle.
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