African Journal of
Cellular Pathology

OFFICIAL PUBLICATION OF THE SOCIETY FOR CELLULAR PATHOLOGY SCIENTISTS OF NIGERIA
  • Abbreviation: Afr. J. Cell. Path
  • Language: English
  • ISSN: 2449-0776
  • DOI: 10.5897/AJCPath
  • Start Year: 2013
  • Published Articles: 80

HIBISCUS EXTACT-IRON-VAN GIESON: A NEW MORPHOLOGICAL STAINING TECHNIQUE IN NEURO-HISTOLOGY

Benard SA
  • Benard SA
  • Histopathology Laboratory , University of Ilorin Teaching Hospital, Ilorin, Kwara State, Nigeria
  • Google Scholar
Afolabi OO
  • Afolabi OO
  • Department of Pathology, College of Health Sciences, University of Ilorin, Ilorin, Kwara State, Nigeria
  • Google Scholar
Olutunde OA
  • Olutunde OA
  • Histopathology Laboratory , University of Ilorin Teaching Hospital, Ilorin, Kwara State, Nigeria
  • Google Scholar


  • Article Number - 184BA3765708
  • Vol.7(5), pp. 30-34 , November 2016
  •  Received: 23 January 2016
  •  Accepted: 23 October 2016
  •  Published: 30 November 2016

Abstract

Aim: To investigate the suitability of Hibiscus extract mordanted with ferric chloride and counterstained with Van Gieson in the demonstration of brain histomorphology.

Method: 10% formol saline fixed brain tissues from cerebellum, cerebrum and basal ganglia were processed by the paraffin wax technique, sectioned and stained with H&E labeled A, Hibiscus/Van Gieson stain labeled B and Weigert’s Van Gieson stain labeled C as control.

Results: Photomicrographs from all the staining techniques presented all the brain cells especially the molecular cell layer and the granular cell layer of the cerebellum in comparable manner. The cells were stained blue-black with the Hibiscus-Van Gieson (B) and Haematoxylin van Gieson (C) methods.

Conclusion: The suitability of Hibiscus extract-Iron-van Gieson in the histomorphological demonstration of brain tissue is established.

Key words: Hibiscus stain, Van Gieson, neuro-histology, roselle.