African Journal of
Cellular Pathology

OFFICIAL PUBLICATION OF THE SOCIETY FOR CELLULAR PATHOLOGY SCIENTISTS OF NIGERIA
  • Abbreviation: Afr. J. Cell. Path
  • Language: English
  • ISSN: 2449-0776
  • DOI: 10.5897/AJCPath
  • Start Year: 2013
  • Published Articles: 60

Haematological changes in layers experimentally infected with salmonella gallinarum

Chiroma Mohammed Adam
  • Chiroma Mohammed Adam
  • Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Maiduguri, Borno State, Nigeria.
  • Google Scholar
Adamu Sani
  • Adamu Sani
  • Department of Veterinary Pathology, Ahmadu Bello University, Zaria, Zaria, Nigeria.
  • Google Scholar
Gadzama Joseph John
  • Gadzama Joseph John
  • Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Maiduguri, Borno State, Nigeria.
  • Google Scholar
Esievo King Akpofure Nelson
  • Esievo King Akpofure Nelson
  • Department of Veterinary Pathology, Ahmadu Bello University, Zaria, Zaria, Nigeria.
  • Google Scholar
Abdulsalam Hassan
  • Abdulsalam Hassan
  • Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Maiduguri, Borno State, Nigeria.
  • Google Scholar
Sani Nuhu Abdulazeez
  • Sani Nuhu Abdulazeez
  • Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Abuja, Abuja, Nigeria.
  • Google Scholar
Joshua Luka
  • Joshua Luka
  • Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri, Nigeria.
  • Google Scholar
Muhammad Ya’u
  • Muhammad Ya’u
  • Department of Animal Health and Production, Binyaminu Usman Polytechnic Hadejia, Jigawa State.
  • Google Scholar


  •  Received: 01 May 2017
  •  Accepted: 01 June 2017
  •  Published: 30 June 2017

Abstract

Aim: The present study was conducted to investigate the haematological changes in layers experimentally infected with Salmonella gallinarum.

Methods: A total of 20 eighteen-week- old ISA Brown layers were used for the experiment. The birds were randomly divided into two groups, infected and control, of 10 birds each. To establish the infection, each bird in the infected group was orally administered 0.5 ml of the inoculum containing 9x108CFU/ml. Similarly, birds in the control group were each administered 0.5 ml normal saline only. Following the inoculation, all experimental birds were closely monitored for clinical signs of fowl typhoid. Blood samples were collected from each group at day zero (Day 0), 2, 4, 7, 14, 21, 28, 35 and 42, post-infection (pi) and used for determination of haematological parameters. By day seven post infection, all birds in the infected group showed clinical signs typical of fowl typhoid; namely weakness, ruffled feathers, huddling together, somnolence, greenish-yellow diarrhea, weight loss, drop in egg production, decrease in feed and water consumption and mortality rate (50%). There were, however, macrocytic hypochromic anaemia, leuckocytosis and heterophilia. In conclusion, the experimental Salmonella Gallinarum infection induced acute anaemia, leukocytosis, heterophilia and lymphopenia.

 

Keywords; Fowl typhoid, Salmonella, Inoculum, Leukocytosis