African Journal of
Cellular Pathology

OFFICIAL PUBLICATION OF THE SOCIETY FOR CELLULAR PATHOLOGY SCIENTISTS OF NIGERIA
  • Abbreviation: Afr. J. Cell. Path
  • Language: English
  • ISSN: 2449-0776
  • DOI: 10.5897/AJCPath
  • Start Year: 2013
  • Published Articles: 65

Full Length Research Paper

Some plasma biochemical changes in layers experimentally infected with Pasteurella multocida

Joseph John Gadzama
  • Joseph John Gadzama
  • Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Maiduguri, Borno State, Nigeria.
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Balami Arhyel Gana
  • Balami Arhyel Gana
  • Department of Veterinary Medicine, University of Maiduguri, Borno State, Nigeria.
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Mohammed Adam Chiroma
  • Mohammed Adam Chiroma
  • Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Maiduguri, Borno State, Nigeria.
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Sani Adamu
  • Sani Adamu
  • Department of Veterinary Pathology, Faculty of Veterinary Medicine, Ahmadu Bello University Zaria, Kaduna State, Nigeria.
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Hassan Abdulsalam
  • Hassan Abdulsalam
  • Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Maiduguri, Borno State, Nigeria.
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Lekko Madaki Yusuf
  • Lekko Madaki Yusuf
  • Department of Veterinary Medicine, University of Maiduguri, Borno State, Nigeria.
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Idoko Sunday Idoko
  • Idoko Sunday Idoko
  • Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Abuja, Nigeria.
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Sani Nuhu Abdulazeez
  • Sani Nuhu Abdulazeez
  • Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Abuja, Nigeria.
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Samson James Enam
  • Samson James Enam
  • Department of Veterinary Pathology, Faculty of Veterinary Medicine, Ahmadu Bello University Zaria, Kaduna State, Nigeria.
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King Akpofure Nelson Esievo
  • King Akpofure Nelson Esievo
  • Department of Veterinary Pathology, Faculty of Veterinary Medicine, Ahmadu Bello University Zaria, Kaduna State, Nigeria.
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  •  Received: 26 May 2018
  •  Accepted: 28 July 2018
  •  Published: 31 August 2018

Abstract

The present study was carried out to investigate some biochemical alterations in layers experimentally infected with Pasteurella multocida. A total of 20 eighteen-week old ISA Brown layers were used in the experiment. The birds were randomly assigned to two groups (infected and control) of 10 layers each. To establish infection, each bird in the infected group was challenged by intra nasal (0.1 ml) and intramuscular (0.4 ml) administration of P. multocida inoculum containing 4.5 × 108 CFU/ml. Meanwhile, birds in the control group were given clean drinking water and fed standard commercial layers mash ad libidum. All the experimental birds were monitored closely for clinical signs of fowl cholera. Blood samples were collected from both groups at day zero (Day 0), 2, 4, 7, 14, 21, 28, 35, 42, post-infection (pi) and used to assay some biochemical parameters. By day 5 post-inoculation (pi), all birds in the infected group manifested clinical signs typical of fowl cholera; weakness, ruffled feathers, sneezing, greenish-yellowish diarrhoea, decrease in feed and water consumption, weight loss, drop in egg production and mortality rate of (20%). However, there were significant increase in the plasma activities of aspartate amino transferase, alanine aminotransferase, alkaline phosphatase, and level of uric acid and significant hypoproteinaemia. The experimental P. multocida infection initiated hepatic, intestinal and renal dysfunctions.

Key words:  Pasteurella multocida, cholera, ISA brown layers.