African Journal of
Cellular Pathology

OFFICIAL PUBLICATION OF THE SOCIETY FOR CELLULAR PATHOLOGY SCIENTISTS OF NIGERIA
  • Abbreviation: Afr. J. Cell. Path
  • Language: English
  • ISSN: 2449-0776
  • DOI: 10.5897/AJCPath
  • Start Year: 2013
  • Published Articles: 65

Isolation and serological detection of mycoplasma gallisepticum and mycoplasma synoviae using a combined mg/ms enzyme-linked immunosorbent assay kit in indigenous chickens in Niger State, Nigeria

Ahmed J S
  • Ahmed J S
  • Central Diagnostic Laboratory, National Veterinary Research Institute Vom, Plateau State Nigeria.
  • Google Scholar
Lawal S M
  • Lawal S M
  • Poultry Unit, A.B.U. Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Ahm adu Bello University, Zaria, Nigeria
  • Google Scholar
Fatihu M Y
  • Fatihu M Y
  • Dept of Veterinary Pathology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
  • Google Scholar
Moses D G
  • Moses D G
  • Central Diagnostic Laboratory, National Veterinary Research Institute Vom, Plateau State Nigeria
  • Google Scholar
Barde I J
  • Barde I J
  • Central Diagnostic Laboratory, National Veterinary Research Institute Vom, Plateau State Nigeria
  • Google Scholar
Kumbish P R
  • Kumbish P R
  • Central Diagnostic Laboratory, National Veterinary Research Institute Vom, Plateau State Nigeria
  • Google Scholar
Oladele S B
  • Oladele S B
  • Dept of Veterinary Pathology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
  • Google Scholar
Ahmed J S
  • Ahmed J S
  • Central Diagnostic Laboratory, National Veterinary Research Institute Vom, Plateau State Nigeria.
  • Google Scholar
Lawal S M
  • Lawal S M
  • Poultry Unit, A.B.U. Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Ahm adu Bello University, Zaria, Nigeria
  • Google Scholar
Fatihu M Y
  • Fatihu M Y
  • Dept of Veterinary Pathology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
  • Google Scholar
Moses D G
  • Moses D G
  • Central Diagnostic Laboratory, National Veterinary Research Institute Vom, Plateau State Nigeria
  • Google Scholar
Barde I J
  • Barde I J
  • Central Diagnostic Laboratory, National Veterinary Research Institute Vom, Plateau State Nigeria
  • Google Scholar
Kumbish P R
  • Kumbish P R
  • Central Diagnostic Laboratory, National Veterinary Research Institute Vom, Plateau State Nigeria
  • Google Scholar
Oladele S B
  • Oladele S B
  • Dept of Veterinary Pathology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
  • Google Scholar


  •  Received: 01 May 2015
  •  Accepted: 01 June 2015
  •  Published: 30 June 2015

Abstract

Aim: The present study was undertaken to determine the seroprevalence of mycoplasma infection and possible isolation from local chickens in Niger State, Nigeria. We have looked into this problem using a combined MG/MS ELISA kit and cultural isolation.

Methods: A total of 552 blood samples were randomly collected from exanguinated chickens for serology. Tracheal swabs were collected into screwed cap bijou bottles containing 2ml of mycoplasma broth medium.

Results: The sero prevalence of indigenous chickens from Niger State was 91.83% by MG/MS Elisa. A total of 126 swabs yielded to the growth of avian mycoplasma on mycoplasma agar.

Key words: Isolation, Mycoplasma species, Avian, indigenous chickens