African Journal of
Cellular Pathology

OFFICIAL PUBLICATION OF THE SOCIETY FOR CELLULAR PATHOLOGY SCIENTISTS OF NIGERIA
  • Abbreviation: Afr. J. Cell. Path
  • Language: English
  • ISSN: 2449-0776
  • DOI: 10.5897/AJCPath
  • Start Year: 2013
  • Published Articles: 64

PRELIMINARY HISTOLOGICAL STUDIES ON THE EFFECT OF AQUEOUS FRUIT EXTRACT OF PHOENIX DACTYLIFERA L. (DATE PALM) ON LEAD ACETATE-INDUCED CEREBELLAR DAMAGES IN WISTAR RATS

Yusuf AO
  • Yusuf AO
  • Department of Human Anatomy, Faculty of Medicine, Ahmadu Bello University, Zaria, Kaduna State, Nigeria
  • Google Scholar
Buraimoh AA
  • Buraimoh AA
  • Department of Human Anatomy, Faculty of Medicine, Kaduna State University, Kaduna, Nigeria
  • Google Scholar
Agbon AN
  • Agbon AN
  • Department of Human Anatomy, Faculty of Medicine, Ahmadu Bello University, Zaria, Kaduna State, Nigeria
  • Google Scholar
Raji K B
  • Raji K B
  • Department of Human Anatomy, Faculty of Medicine, Ahmadu Bello University, Zaria, Kaduna State, Nigeria
  • Google Scholar
Akpulu PS
  • Akpulu PS
  • Histology Unit, Department of Human Anatomy, Faculty of Medicine, Ahmadu Bello University, Zaria, Kaduna State, Nigeria
  • Google Scholar


  •  Received: 01 December 2016
  •  Accepted: 01 January 2017
  •  Published: 31 January 2017

Abstract

Aim: This study was to histologically assess the therapeutic effect of aqueous fruit extract of Phoenix dactylifera (AFEPD) on lead acetate-induced cerebellar damage in Wistar rats.

Methods: Twenty four rats were grouped into six (I–VI; n=4). Group I (control) received distilled water (1 ml/kg). Group II received lead acetate (LA, 120mg/kg) only. Groups III and IV received LA (120mg/kg) followed by AFEPD (1000mg/kg and 1500mg/kg, respectively). Groups V and VI received AFEPD (1000mg/kg and 1500mg/kg, respectively). All administrations were by oral route. Treatment lasted 28 days; LA was administered from day 1 to day 14, while AFEPD was administered from day 15 to day 28 of the experimental period. Therapeutic activity of AFEPD was assessed by histological examination of the cerebellar cortex with H and E stain.

Results: Findings revealed neurodegenerative changes in the cerebellar cortex like perineuronal vacoulations and cytoplasmic shrinkage in molecular layer cells and Purkinje cells in LA-intoxicated group. The administration of AFEPD remarkably ameliorated LA–induced cerebellar damage dose dependently. Normal cerebellar histoarchitecture was observed with administration of AFEPD only.

Conclusion: Results suggest that AFEPD has therapeutic potentials against lead acetate-induced cerebellar damage in Wistar rats.

 

Key words: Cerebellum, Lead acetate, Phoenix dactylifera