African Journal of
Cellular Pathology

OFFICIAL PUBLICATION OF THE SOCIETY FOR CELLULAR PATHOLOGY SCIENTISTS OF NIGERIA
  • Abbreviation: Afr. J. Cell. Path
  • Language: English
  • ISSN: 2449-0776
  • DOI: 10.5897/AJCPath
  • Start Year: 2013
  • Published Articles: 65

Full Length Research Paper

Determination of median lethal dose of ethanolic extract of Sesamum Indicum seeds in adult wistar rats

Yusuf M.K.
  • Yusuf M.K.
  • Department of Human Anatomy, Faculty of Medicine, Ahmadu Bello University Zaria, Nigeria.
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Ibegbu AO
  • Ibegbu AO
  • Department of Human Anatomy, Faculty of Medicine, Ahmadu Bello University Zaria, Nigeria.
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Agbon AN
  • Agbon AN
  • Department of Human Anatomy, Faculty of Medicine, Ahmadu Bello University Zaria, Nigeria.
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Evang A
  • Evang A
  • Department of Human Anatomy, Faculty of Medicine, Ahmadu Bello University Zaria, Nigeria.
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  •  Received: 01 November 2017
  •  Published: 31 December 2017

Abstract

The aim of this research was to determine the median lethal dose of sesamum
indicum seeds in Wistar rats.
Methods: Twelve Wistar rats within the ages of five to six weeks and of weights, ranging from (98-160) g of both sexes were used for the experiment. They were categorized into two phases of I and II. Phase I consisted three (3) groups of three
(3) animals each. While phase II consisted three (3) groups of one (1) animal each. The phase I group was orogastrically intubated with (10, 100 and 1000) mg/kg body weight of ethanolic extract of sesamum indicum seeds respectively with the aid of a 2ml syringe with an intubation needle of size 24 mounted on it. The animals in phase II were similarly administered 1600, 2900 and 5000mg/kg body weights of the extract respectively and observed for one hour and ten minutes after every two hours for twenty four hours.
Result: No mortality or any physical signs of toxicity were observed in any of the groups.
Conclusion: The LD50 of ethanolic extract of sesamum indicum seeds is above 5000mg/kg body weight in adult Wistar rats.
 
Key Words: LD50, Sesamum seeds, Wistar rats.