African Journal of
Cellular Pathology

OFFICIAL PUBLICATION OF THE SOCIETY FOR CELLULAR PATHOLOGY SCIENTISTS OF NIGERIA
  • Abbreviation: Afr. J. Cell. Path
  • Language: English
  • ISSN: 2449-0776
  • DOI: 10.5897/AJCPath
  • Start Year: 2013
  • Published Articles: 65

Sorghum bicolor extract: a suitable counter stain in hibiscus extract nuclear staining of liver and kidney

Benard SA
  • Benard SA
  • Histopathology Unit, Department of Pathology, University of Ilorin Teaching Hospital, Ilorin, Kwara State, Nigeria
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Muhammed AO
  • Muhammed AO
  • Department of Histopathology, Faculty of Medical Laboratory Science, Usman Danfodiyo University, Sokoto, Sokoto State, Nigeria
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Fowotade AA
  • Fowotade AA
  • Histopathology Unit, Department of Pathology, University of Ilorin Teaching Hospital, Ilorin, Kwara State, Nigeria
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Afolabi OO
  • Afolabi OO
  • Department of Pathology, College of Health Sciences, University of Ilorin, Ilorin, Kwara State, Nigeria
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Olutunde OA
  • Olutunde OA
  • Histopathology Unit, Department of Pathology, University of Ilorin Teaching Hospital, Ilorin, Kwara State, Nigeria
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  •  Received: 01 January 2015
  •  Accepted: 01 February 2015
  •  Published: 28 February 2015

Abstract

Aim: To investigate the suitability of alcoholic extract of Sorghum bicolor stalk as a counter stain for Hibiscus extract stained nuclei. Method: Formalin fixed kidney and liver biopsies were processed by the paraffin wax technique, sectioned, stained with Hibiscus extract solution and counterstained with 10% alcoholic extract of Sorghum bicolor stalk. Parallel sections were stained with H&E as control.

Results: Results show distinct dark - violet nuclear staining with light-brown staining of collagen and other components.

Conclusion: Sorghum bicolor stalk alcoholic extract could be a useful substitute to eosin in histology for the demonstration of collagen and other cytoplasmic components especially when H. sabdariffa is used as the nuclear stain.

Keywords: Hibiscus, Nuclear staining, Sorghum bicolor extract