Biotechnology and Molecular Biology Reviews

  • Abbreviation: Biotechnol. Mol. Biol. Rev.
  • Language: English
  • ISSN: 1538-2273
  • DOI: 10.5897/BMBR
  • Start Year: 2006
  • Published Articles: 102


Developmental competence of in vivo and in vitro matured oocytes: A review

Blanco M. R1*, Demyda S.1, Moreno Millán  M.2 and  Genero E1
1Agricultural Sciences Faculty, Lomas de Zamora National University. Ruta 4, Km. 2, (1836) Llavallol, Buenos Aires, Argentina. 2Department of Genetic, University of Cordoba, Spain.
Email: [email protected]

  •  Accepted: 19 August 2011
  •  Published: 30 September 2011


The study of follicular dynamics has been particularly rapid in the last two decades. However,in vitro maturation and fertilization of oocytes results in reduced embryo production, which suggests that not all oocytes have the capacity to mature and to fertilize properly. The efficiency of bovine embryo production in vitro, measured as the blastocyst rate, obtained from oocytes matured and fertilized in vitro, is about 40%. Evidence has demonstrated that oocyte quality depends on the events that occur before germinal vesicle breakdown (GVBD) and has suggested that the oocyte must accumulate the appropriate information for meiotic resumption, fertilization and early embryonic development before chromosome condensation. The reduced blastocyst yield obtained in vitro seems to be related to oocyte source and quality, and the culture system applied. The oocyte quality and protocols of culture are the key factors that determine embryo development, establish a pregnancy and produce healthy offspring.


Key words: Oocyte, in vitro maturation (IVM), vitro fertilization (IVF), in vitro culture (IVC), embryo, bovine.