Abstract
Chlorophytum borivilianum (Family: Liliaceae), an important threatened medicinal herb is designated as ‘Rare’ in Red Data Book of Indian plants. In this study, random amplified polymorphic primer DNA (RAPD) markers were used to assess the genetic diversity in six genotypes of C. borivilianum collected from different geographical regions of Rajasthan, Madhya Pradesh and Jharkhand. Out of the 86 bands, 45 were found to be polymorphic and the level of polymorphism was 52.35% thus, revealing a high degree of polymorphism. The maximum polymorphism was obtained using primer number 108 that was 90.90% and least with primer number 102 that was 12.5%. The Jaccard’s similarity coefficient ranged from 0.70 ± 0.186. The maximum similarity value was noticed between SM3 and SM5 genotypes collected from KRD (M.P) and Maa Umaya farm (M.P.), respectively.
Key words: Chlorophytum borivilianum, genetic diversity, random amplified polymorphic primer DNA (RAPD), genotypes.