International Journal of
Biodiversity and Conservation

  • Abbreviation: Int. J. Biodivers. Conserv.
  • Language: English
  • ISSN: 2141-243X
  • DOI: 10.5897/IJBC
  • Start Year: 2009
  • Published Articles: 687

Full Length Research Paper

Genetic variation of striped snakehead (Channa striatus Bloch, 1793) populations using random amplified polymorphic DNA (RAPD) markers

Ajaz Ali Bhat
  • Ajaz Ali Bhat
  • Centre for Aquaculture Research and Extension, St. Xavier’s College, Palayamkottai Tamilnadu-627 002, India.
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M. A. Haniffa*
  • M. A. Haniffa*
  • Centre for Aquaculture Research and Extension, St. Xavier’s College, Palayamkottai Tamilnadu-627 002, India.
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M. James Milton
  • M. James Milton
  • Centre for Aquaculture Research and Extension, St. Xavier’s College, Palayamkottai Tamilnadu-627 002, India.
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Bilal Ahmad Paray
  • Bilal Ahmad Paray
  • Centre for Aquaculture Research and Extension, St. Xavier’s College, Palayamkottai Tamilnadu-627 002, India.
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P. R. Divya
  • P. R. Divya
  • National Bureau of Fish Genetic Resources (NBFGR), Cochin Unit, CMFRI Campus, Kochi- 682 018, India.
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A. Gopalakrishnan
  • A. Gopalakrishnan
  • National Bureau of Fish Genetic Resources (NBFGR), Cochin Unit, CMFRI Campus, Kochi- 682 018, India.
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  •  Received: 02 November 2013
  •  Accepted: 15 April 2014
  •  Published: 28 May 2014

Abstract

Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was applied to analyze the genetic variation among Channa striatus collected from four geographically distant locations in India. After initial PCR screening, nine random oligodecamers viz. OPA10, OPA11, OPA15, OPAC01, OPAC03, OPAC05, OPAC07, OPAC09 and OPAC19 which generated the RAPD profile for the four C. striatus populations were selected. Amplification using these nine primers resulted in fragments ranging in length between 309-3029 bp assigned to 87 loci. Estimates of Nei’s (1978) unbiased genetic distance (D) values ranged from 0.3242-0.6320. Unweighted pair group method with arithmetic mean (UPGMA) dendrogram constructed on the basis of genetic distance revealed very close genetic relationship among C. striatus populations of river Tamirabarani (Tamil Nadu) and river Periyar (Kerala). Both populations from river Tamirabarani and Periyar were found to be genetically closer to fish populations from Kolleru Lake (Andhra Pradesh) whereas C. striatus population from Brahmani River (Orissa) was found to be genetically distant from the rest of the populations.

Key words: Indian snakehead, Channa striatus, genetic diversity, random amplified polymorphic DNA (RAPD).

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This article is published under the terms of the Creative Commons Attribution License 4.0

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