International Journal of
Medicine and Medical Sciences

  • Abbreviation: Int. J. Med. Med. Sci.
  • Language: English
  • ISSN: 2006-9723
  • DOI: 10.5897/IJMMS
  • Start Year: 2009
  • Published Articles: 535

Full Length Research Paper

Standardization of the method to obtain therapeutic-quality platelet-rich plasma

Aurora Martínez-Romero*
  • Aurora Martínez-Romero*
  • Facultad de Ciencias Químicas Unidad Gómez Palacio, Universidad Juárez del Estado de Durango (UJED), México
  • Google Scholar
José Luis Ortega-Sánchez
  • José Luis Ortega-Sánchez
  • Unidad Regional Universitaria de Zonas Áridas, Universidad Autónoma Chapingo, Bermejillo, Durango, México.
  • Google Scholar
Reyna Margarita Hernández-Ramos
  • Reyna Margarita Hernández-Ramos
  • Facultad de Ciencias Químicas Unidad Gómez Palacio, Universidad Juárez del Estado de Durango (UJED), México
  • Google Scholar
José Prospero Hernández-de-la-Fuente
  • José Prospero Hernández-de-la-Fuente
  • Sanatorio San José, Gómez Palacio, Durango, México
  • Google Scholar
Maribel Cervantes-Flores
  • Maribel Cervantes-Flores
  • Facultad de Ciencias Químicas Unidad Durango, UJED, México
  • Google Scholar
Norma Urtiz-Estrada
  • Norma Urtiz-Estrada
  • Facultad de Ciencias Químicas Unidad Durango, UJED, México
  • Google Scholar
Estela Ruiz-Baca
  • Estela Ruiz-Baca
  • Facultad de Ciencias Químicas Unidad Durango, UJED, México
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José de Jesús Alba-Romero
  • José de Jesús Alba-Romero
  • Sanatorio San José, Gómez Palacio, Durango, México
  • Google Scholar


  •  Received: 29 October 2013
  •  Accepted: 03 March 2014
  •  Published: 30 April 2014

Abstract

Platelet-rich plasma (PRP) is a reliable source for obtaining cells to regenerate tissues, with ease of availability inorder to implement and standardize the ideal methodology in centrifugation strength and time for obtaining therapeutic-quality PRP, allowing its application to provide better and rapid recovery of muscular injuries, tendinitis, bone and ligament lesions. To evaluate PRP therapy, 150 patients with muscular lesions, tendinitis, shoulder, knee, ankle, hand and elbow injuries were treated. On application of PRP, we obtained 100% clinically significant symptomatic improvement in all 150 patients treated, who had musculoskeletal and ligament injuries, with a marked reduction of pain and inflammation. We concluded that the ideal concentration for obtaining PRP is at 1000 rpm with a time of 5 min; in addition, under these conditions the plasma lacks leukocytes and erythrocytes. The results were reproducible because the experiment was repeated at two institutions under the same conditions and similar results were obtained. The regeneration obtained in the affected patients is due to the fact that growth factors were released from the activated platelets; these initiate and modulate cicatrization in the tissues, which is a recent innovation to promote cicatrization, accelerating the power of tissue regeneration, with a platelet concentrate suspended in plasma.

 

Key words: Growth factors, platelet activation, application, tissue regeneration, therapeutic quality.