January 2012

Comparative study of different protocols for isolation of RNA from polysaccharides and polyphenolics rich plant tissues

The isolation of high quality ribonucleic acid (RNA) from plant has been a difficult problem due to presence of high amount of polysaccharides, polyplenolics and nucleases that mediate RNA degradation. After comparative analysis of four popular protocols for RNA isolation, a modified method was developed which gave maximum yield with purity. Modified method is not only suitable for the isolation of intact RNA from...

Author(s): Subodh Gupta, A. K. Verma and Laxmi Chand

January 2012

Abundant CD4 Th-2 cytokine stimulation by medicinal plant Pongamia pinnata Linn. on human peripheral blood mononuclear cell (PBMC)

Immune cells produce cytokines in response to various stimuli. The initial cytokine milieu at which immune cells interact have a positive impact on the outcome of the immune response generated. Modulations or polarizations of cytokines are known to affect the outcome of the disease. Immunotherapy is a novel approach to combat cytokine mediated diseases and several medicinal plants are shown to possess immune modulatory...

Author(s): M. Manikannan, P. Durgadevi, S. Subramaniyan and Elanchezhiyan Manickan