International Journal of
Physical Sciences

  • Abbreviation: Int. J. Phys. Sci.
  • Language: English
  • ISSN: 1992-1950
  • DOI: 10.5897/IJPS
  • Start Year: 2006
  • Published Articles: 2490

Full Length Research Paper

Spatial distributions and energy landscape of MinE protein dynamics via the biophysical spot tracking technique

Sitta Aroonnual1, Waipot Ngamsaad1,7, Paisan Kanthang1,2, Narin Nuttawut1,6, Wannapong Triampo1,4,6*, Darapond Triampo3,6  and Chartchai Krittanai5
1R&D Group of Biological and Environmental Physics, Department of Physics, Faculty of Science, Mahidol University, Bangkok 10400, Thailand. 2Rajamangala University of Technology, Phra Nakhon, Bangkok 10800, Thailand. 3Department of Chemistry, Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand. 4Institute for Innovative Learning, Mahidol University, 999, Phuttamonthon 4 Road, Salaya, Nakorn Pathom 73170, Thailand. 5Institute of Molecular Biosciences, Mahidol University, Salaya Campus, Nakhon Pathom, Thailand 6ThEP Center, CHE, 328 Si Ayutthaya Road, Bangkok, 10400 Thailand. 7School of Science, University of Phayao, Phayao 56000, Thailand
Email: [email protected]

  •  Accepted: 02 June 2011
  •  Published: 04 August 2011

Abstract

The MinCDE protein system is known to dictate cytokinesis cell division in prokaryotes by spatial regulation of the Z-ring. The oscillatory dynamics of MinC and MinD depends on the presence of MinE, where the MinE protein dynamics acts as a topological specificity to the midcell. In this work, the Spot Tracking Technique is used to determine the biophysical quantities of MinE protein dynamics, namely, diffusive motion, velocity distribution, spatial distribution, and energy profile. An alternative quantity that indicates the potential of the mean force characteristic function is proposed to be an effective potential parameter to indicate the optimal energy to generate a stable spatial-temporal pattern formation of MinE proteins. The localization and distribution patterns along the cell length were well confirmed, while other quantitative information related to MinE cluster positions have been revealed. In addition, the effective potential was found to relate to the spring-like potential. The minimum region indicates the potential cluster depth that occurs near the midcell zone, which corresponds to the finding that the MinE cluster is mostly concentrated at midcell.

 

Key words: Spot tracking technique, cell division, MinE proteins, protein oscillation.

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