Abstract

This paper investigates the application of Fresnel based numerical algorithms for the reconstruction of Gabor in-line holograms. A simple in-line digital holographic system was used to record blood cell. The Fresnel transformation (FS) method was used for reconstruction of the hologram. High contrast blood cell images were used to demonstrate numerical reconstruction process by Matlab. Sine, cosin, and amplitude holograms were reconstructed. This method provides new insights into the dynamics of the red blood cells and will be further used to investigate the effect of physiological and pharmacological effectors on RBCs. The spontaneous cell membrane fluctuations (CMF) of the red blood cell (RBC) was investigated, by calculating both thickness and refraction index of the cell blood.

Key words: Digital holography, blood cell, refractive index.