International Journal of
Physical Sciences

  • Abbreviation: Int. J. Phys. Sci.
  • Language: English
  • ISSN: 1992-1950
  • DOI: 10.5897/IJPS
  • Start Year: 2006
  • Published Articles: 2569

Full Length Research Paper

Production and purification of horseradish peroxidase in Pakistan

Shazia Khurshid1*, Muhammad Zahid Qureshi1, Abida Ibrahim1, Zahid Nawaz1, Amina Sajid1 and Muhammad Ikram2
  1Department of Chemistry, GC University, Lahore, Pakistan. 2FCC Charted University, Lahore, Pakistan.
Email: [email protected]

  •  Accepted: 10 April 2012
  •  Published: 31 May 2012

Abstract

 

Horseradish peroxidase is heme containing enzyme belonging to oxidoreductases and is involved in degradation of certain recalcitrant organic compounds like phenol and substituted phenol via free radical polymerization. Peroxidase was extracted and purified from horseradish by (NH4)2SO4 precipitation, dialysis and gel filtration chromatography. Spectrophotometer was used for the assay of horseradish peroxidase in this present project. Horseradish peroxidase increased activity from 6.3027 to 9.9452 U/ml by purification. It gained 45.77 folds purification at this final step. It is helpful in determination of glucose in blood and compounds in it kill bacterial strains.

 

Key words: Horseradish, peroxidase, heme, oxidoreductase, activity.