Abstract

Horseradish peroxidase is heme containing enzyme belonging to oxidoreductases and is involved in degradation of certain recalcitrant organic compounds like phenol and substituted phenol via free radical polymerization. Peroxidase was extracted and purified from horseradish by (NH₄)₂SO₄ precipitation, dialysis and gel filtration chromatography. Spectrophotometer was used for the assay of horseradish peroxidase in this present project. Horseradish peroxidase increased activity from 6.3027 to 9.9452 U/ml by purification. It gained 45.77 folds purification at this final step. It is helpful in determination of glucose in blood and compounds in it kill bacterial strains.

Key words: Horseradish, peroxidase, heme, oxidoreductase, activity.