Abstract

The application of spectroscopy for the study of biomedical compounds has increased tremendously in recent years. Blood is the chief circulatory medium in human and in animal body which can be subjected to non-invasive technique for testing. Pre and post vaccinated blood samples of sheep vaccinated with anthrax spore vaccine was studied using spectrometer. The internal standards among the application peaks were calculated. There was a marked difference in the absorption levels of the pre and post vaccinated blood samples. The resultant variation was attributed to the chemical changes that happened in the animal blood due to the vaccination. Spectral study can emerge as an alternate and cost effective test for screening animals. In future this study can be extended and compared with other antibody tests like ELISA (Enzyme-Linked Immuno Sorbent Assay).

Key words: Spectroscopy, anthrax spore vaccine, antibody, ELISA (Enzyme-Linked Immuno Sorbent Assay).