International Journal of
Physical Sciences

  • Abbreviation: Int. J. Phys. Sci.
  • Language: English
  • ISSN: 1992-1950
  • DOI: 10.5897/IJPS
  • Start Year: 2006
  • Published Articles: 2557

Full Length Research Paper

Structure elucidation of three new diterpene glycosides from Stevia rebaudiana

Venkata Sai Prakash Chaturvedula* and Indra Prakash
The Coca-Cola Company, Organic Chemistry Department, Global Research and Development, One Coca-Cola Plaza, Atlanta, GA 30313, United State America.
Email: [email protected]

  •  Accepted: 13 October 2011
  •  Published: 16 November 2011



Three new minor diterpene glycosides were isolated from the commercial extract of the leaves of Stevia rebaudiana and their structures were identified as 13-[(2-O-b-D-glucopyranosyl-b-D-glucopyranosyl)oxy] ent-kaur-15-en-19-oic acid b-D-glucopyranosyl ester (1),13-[(2-O-b-D-glucopyranosyl-3-O-b-D-glucopyranosyl-b-D-glucopyranosyl)oxy]ent-kaur-15-en-19-oic acid b-D-glucopyranosyl ester (2) and 13-[(2-O-b-D-glucopyranosyl-3-O-b-D-glucopyranosyl]- b-D-glucopyranosyl)oxy] 17-hydroxy-ent-kaur-15-en-19-oic acidb-D-glucopyranosyl ester based on extensive spectroscopic data and chemical studies.


Key words: Stevia rebaudiana, compositae, asteraceae, diterpene glycosides, structural characterization, spectral data, acid and enzymatic hydrolysis.



IR, Infrared; FT-IR, fourier transform infrared; UATR, universal attenuated total reflectance; NMR, nuclear magnetic resonance; MS, mass spectrometer; Q-Tof, quadrupole time-of-flight; HPLC, high performance liquid chromatography; TFA,trifluoroacetic acid; UV, ultraviolet; HPLC, high performance liquid chromatography mass spectrometer; HRMS, high resolution mass spectral data.