Full Length Research Paper
Abstract
Three new minor diterpene glycosides were isolated from the commercial extract of the leaves of Stevia rebaudiana and their structures were identified as 13-[(2-O-b-D-glucopyranosyl-b-D-glucopyranosyl)oxy] ent-kaur-15-en-19-oic acid b-D-glucopyranosyl ester (1),13-[(2-O-b-D-glucopyranosyl-3-O-b-D-glucopyranosyl-b-D-glucopyranosyl)oxy]ent-kaur-15-en-19-oic acid b-D-glucopyranosyl ester (2) and 13-[(2-O-b-D-glucopyranosyl-3-O-b-D-glucopyranosyl]- b-D-glucopyranosyl)oxy] 17-hydroxy-ent-kaur-15-en-19-oic acidb-D-glucopyranosyl ester based on extensive spectroscopic data and chemical studies.
Key words: Stevia rebaudiana, compositae, asteraceae, diterpene glycosides, structural characterization, spectral data, acid and enzymatic hydrolysis.
Abbreviation
IR, Infrared; FT-IR, fourier transform infrared; UATR, universal attenuated total reflectance; NMR, nuclear magnetic resonance; MS, mass spectrometer; Q-Tof, quadrupole time-of-flight; HPLC, high performance liquid chromatography; TFA,trifluoroacetic acid; UV, ultraviolet; HPLC, high performance liquid chromatography mass spectrometer; HRMS, high resolution mass spectral data.
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