African Journal of
Bacteriology Research

  • Abbreviation: J. Bacteriol. Res.
  • Language: English
  • ISSN: 2006-9871
  • DOI: 10.5897/JBR
  • Start Year: 2009
  • Published Articles: 103

Full Length Research Paper

Isolation and identification of Mannhemia and Pasturella species from pneumonic and apparently healthy cattle and their antibiogram susceptibility pattern in Bedelle District, Western Ethiopia

Dereje Abera
  • Dereje Abera
  • School of Veterinary Medicine, College of Medical and Health Sciences, Wollega University, P.O. Box 395, Nekemte, Ethiopia.
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Tesfaye Sisay
  • Tesfaye Sisay
  • Unit of Health Biotechnology, Institute of Biotechnology, College of Natural and Computational Sciences, Addis Ababa University, P.O.Box 34, Debre Zeit, Ethiopia.
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Tadesse Birhanu*
  • Tadesse Birhanu*
  • School of Veterinary Medicine, College of Medical and Health Sciences, Wollega University, P.O. Box 395, Nekemte, Ethiopia.
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  •  Received: 01 October 2014
  •  Accepted: 06 December 2014
  •  Published: 31 December 2014

Abstract

The study was conducted from October 2012 to April 2013 at Bedelle district, Western Ethiopia. The aim was to isolate Mannheimia and Pasteurella species, and to assess the associated risk factors from pneumonic and apparently healthy cattle, and antibiotic susceptibility profiles of the isolates. Out of 329 samples from animals (185 nasal swabs from clinic and 144 lung tissues abattoir) examined, 50.2% was found to be positive to the disease. The bacteriological examination revealed 28 (8.51%) overall isolates of Mannheimia haemolytica (46.4%), Pasteurella multocida (39.3%) and Bibersteina trehalosi (14.3%) were recovered respectively in which 17 (9.19%) and 11 (7.63%) bacterial isolates were obtained from nasal swabs and pneumonic lungs, respectively. The higher isolation rate of M. haemolytica indicated it as the major cause in the study area. Age was found to be the potential risk factor in which young animals were highly affected. The antimicrobial susceptibility profiles of the isolates were carried out using disc diffusion method. The isolates were susceptible to most of the antibiotic disks used: amoxicillin, chloramphenicol, cephalexin, polymyxin-B, kanamycin and florifenicol. However, moderate resistance was observed to Tetracycline, Erythromycin and Penicillin-G. Thus, an integrated application of overall management and vaccination should be implemented as prevention and control measures.

 

Key words: Bedelle, cattle, Mannheimia, Pasteurella, pneumonic pasteurellosis, antimicrobial susceptibility test.