One hundred twenty patients with chronic suppurative otitis media (CSOM) in Basrah, 65(54.2%) males and 55(45.8%) Females, with male: females ratio (1.2:1) and 60 individual without ontological problems as control group were included in this study, which was done during the period of March, 2009 to January, 2010. This Include collection of aural swab samples, culturing of samples, identification of causative agents species and antibiotic sensitivity. Gram's negative bacteria were the commonest microorganisms; it comprises (60%). Pseudomonas aeruginosa was common causative agent (19.04%), followed by Staphylococcus aureus (16.7%) and Klebsiella spp. (14.3%). Mixed infection was found in high percent (74%), in which P. aeruginosa and other microorganisms were more common. The antibiotic sensitivity pattern showed that P. aeruginosa was sensitive to Ciprofloxacin, amoxicillin +clavulanic acid and gentamicin, while other is appeared resistant, S. aureus was sensitive to ciprofloxacin, moxicillin+clavulanic acid, erthomycin, cephalexine and it is resistant to pencillin and ampicillin, klebsiella species were sensitive to ciprofloxacin, amoxicillin +clavulanic acid ,gentamicin,while resistant to tetracycline.
Key words: Antibiotics, bacteria, otitis media.