African Journal of
Bacteriology Research

  • Abbreviation: J. Bacteriol. Res.
  • Language: English
  • ISSN: 2006-9871
  • DOI: 10.5897/JBR
  • Start Year: 2009
  • Published Articles: 113

Full Length Research Paper

Multidrug resistant bacteria isolated from nosocomial infections at University Teaching Hospital of Point-G, Bamako, Mali

Maiga Aminata
  • Maiga Aminata
  • Medical Biology and Hospital Hygiene Laboratory, Point-G University Hospital, Bamako, Mali.
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Beye Seydina Alioune
  • Beye Seydina Alioune
  • Faculty of Medicine and Odontostomatology, University Teaching Hospital of Point-G, Bamako, Mali.
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Cissoko Yacouba
  • Cissoko Yacouba
  • Faculty of Medicine and Odontostomatology, University Teaching Hospital of Point-G, Bamako, Mali.
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Dicko Oumar Agaly
  • Dicko Oumar Agaly
  • Medical Biology and Hospital Hygiene Laboratory, Point-G University Hospital, Bamako, Mali.
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Diarra Bassirou
  • Diarra Bassirou
  • Faculty of Medicine and Odontostomatology, University Teaching Hospital of Point-G, Bamako, Mali.
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Traoré Abdoulaye
  • Traoré Abdoulaye
  • National Institute for Training in Health Sciences, Mali.
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Coulibaly Djibril Mamadou
  • Coulibaly Djibril Mamadou
  • Medical Biology and Hospital Hygiene Laboratory, Point-G University Hospital, Bamako, Mali.
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Koné Drissa
  • Koné Drissa
  • Medical Biology and Hospital Hygiene Laboratory, Point-G University Hospital, Bamako, Mali.
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Diarra Lobogal
  • Diarra Lobogal
  • Medical Biology and Hospital Hygiene Laboratory, Point-G University Hospital, Bamako, Mali.
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Coulibaly Djaminatou
  • Coulibaly Djaminatou
  • Medical Biology and Hospital Hygiene Laboratory, Point-G University Hospital, Bamako, Mali.
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Coulibaly Youssouf
  • Coulibaly Youssouf
  • Faculty of Medicine and Odontostomatology, University Teaching Hospital of Point-G, Bamako, Mali.
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Maiga Ibrahim Izetiégouma
  • Maiga Ibrahim Izetiégouma
  • Medical Biology and Hospital Hygiene Laboratory, Point-G University Hospital, Bamako, Mali.
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Fofana Djeneba Bocar
  • Fofana Djeneba Bocar
  • Faculty of Medicine and Odontostomatology, University Teaching Hospital of Point-G, Bamako, Mali.
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  •  Received: 18 October 2022
  •  Accepted: 13 December 2022
  •  Published: 31 January 2023

Abstract

An infection is said to be nosocomial or hospital if it is absent when the patient enters the hospital and it appears and develops at least 48 h late. The objective of this study was to determine the resistance phenotypes of bacteria isolated from nosocomial infections at the University Teaching Hospital of Point G. Urine, blood, pus, skin and bronchoalveolar fluid samples were taken in different units, and bacteria isolations were performed on usual selective media such as Drigalski Colombia agar supplemented with nalidixic acid and colistin and 5% sheep blood and chocolate agar. Identifications of bacteria such as Enterobacteriaceae, Pseudomonas and acinetobacter, and Staphylococci were done using API20E gallery, API20NE gallery and catalase/oxidase tests, and the Pastorex Staph kit respectively. The antimicrobial susceptibility testing was performed on Mueller-Hinton agar using the diffusion method. A total of 463 patients were inpatients for at least 48 h in the different units, and a nosocomial infection was notified in at least 57 patients (12.3%). A total of 65 episodes of nosocomial infections were observed in these 57 patients. Of the bacteria isolated, multidrug-resistant bacteria (MDR) represented 63.7% (n=36). These were extended-spectrum beta-lactamase (ESBL)-secreting Enterobacteriaceae (n=21), high-level cephalosporinase (n=13) and methicillin-resistant coagulase-negative Staphylococci (n=2). Despite this high number of multi-resistant bacteria isolated in this study; colistin and amikacin had very good activity on enterobacteriaceae. The results show the need to strengthen hygiene in the intensive care units in order to fight against nosocomial infections at the UTH of Point G.

 

Key words: Nosocomial infections, multi-resistant bacteria, UTH of Point-G.