African Journal of
Bacteriology Research

  • Abbreviation: J. Bacteriol. Res.
  • Language: English
  • ISSN: 2006-9871
  • DOI: 10.5897/JBR
  • Start Year: 2009
  • Published Articles: 104

Full Length Research Paper

A potential new isolate for the production of a thermostable extracellular α- amylase

Ahmed, A. Alkando, Hanan Moawia Ibrahim*
Central Laboratory, Ministry of Science and Technology, Sudan.
Email: [email protected]

  • Article Number - 4E7C0499813
  • Vol.3(8), pp. 129-137, October 2011
  •  Accepted: 15 July 2011
  •  Published: 31 October 2011

Abstract

 

Two hundred and seventy Bacillus species were isolated from soil samples in Khartoum State and tested for α- amylase production. 20 potential isolates of α- amylase producer were obtained during primary screening. Secondary screening of these isolates yielded a high thermostable alkaline α- amylase producing isolate. Of all the species tested, Bacillus licheniformis gave maximum α-amylase activity of 0.7947 U/mg/ml at pH of 8 in iodine method, and (0.024 U/mg/ml) in 3.5.dinitrosalicylic acid (DNS) method. Characterization of the extra-cellular crude α- amylase was further evaluated for its biochemical properties as an enzyme for industrial use. The production of α- amylase following growth of the microorganism was found to be at optimum temperature and pH of 70°C and 9.0, respectively.

 

Key words: Thermostable α- amylase, alkaline α- amylase, Bacillus licheniformis.

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