African Journal of
Bacteriology Research

  • Abbreviation: J. Bacteriol. Res.
  • Language: English
  • ISSN: 2006-9871
  • DOI: 10.5897/JBR
  • Start Year: 2009
  • Published Articles: 103

Full Length Research Paper

The presence of cagA genome in blood of infected patient with Helicobacter pylori as new marker

Hatami saeideh1, Esmaeili Davoud2*, Fallah Jali l.2 and Rezaee Jafar2
  1Science and Research Branch, Islamic Azad University, Department of Biology Tehran, Iran. 2Applied Microbiology Research center and Department of Microbiology, Faculty of Medical science, Baqyiatallah University, Tehran, Iran.
Email: [email protected]

  •  Accepted: 03 April 2013
  •  Published: 30 April 2013

Abstract

 

Helicobacter pylori is a motile and gram negative bacterium, that has been associated with gastritis, peptic ulcer, and gastric adenocarcinoma. The strains ofHelicobacter pylori contain CagA associated with severe infections. To investigate the presence of cagA gene in Helicobacter pylori strains among patients’ sera, about 100 serum specimens that had positive antibody against H. pylori were collected. Serum samples were studied using designed primers. The scale of triglyceride and cholestrol serum samples was measured. PCR method was done on sera of H. pylori samples that had antibody titers against H. pylori .The results indicated that 18% from all the serum samples contain cagA DNA. This study indicates that 12 serum samples contain triglyceride and cholesterol (TG & Chol) titers higher than normal scale. According to the findings, it is suggested that the presence of CagA DNA in strains of Helicobacter pylori may play an important role in aggravating disorders. In this investigation we were able to detect H. pylori cagA DNA in patients’ sera. H. pylori probably attaches to red blood cell and causes bacteremia, but is also able to secrete CagA protein and cagA DNA to host cells by T4 secretory system. Thus, there is risk of integrating cagA DNA in host cell chromosome and inducting antibodies against it. Between the presence of cagA DNA and increase of triglyceride and cholesterol, there exists rational relation.

 

Key words: Detection, cagA genome, blood, marker.

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