To perform moleular detection of Chlamydia trachomatis among gynecological patients attending Khartoum Teaching Hospital. 200 endocerical smears were collected randomly from out-patient infertile women attending the Department of Obsetrcs and Gyencology, Khartoum Teaching Hospital (Sudan). Detection of C. trachomatis was performed by the polymerase chain reaction (PCR) technique. About 191 out of 200 patients having vaginal discharge were investigated, 43 (22.5%) of them were found positive for C. trachomatis by PCR test. Most positive cases were in the age range 26-40 years, whereas negative cases were in the age range 61 years and above. The age range (20-40) years, is a risk factor that exposes sexually-active women to C. trachomatis infection. Also, C. trachomatis infection in pregnant women may be associated with abortion and pelvic inflammatory disease.C. trachomatis infection is becoming a public health problem in Khartoum due to its highest frequency among infertile Sudanese women.
Key words: Chlamydia trachomatis, polymerase chain reaction, Sudanese women.
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