African Journal of
Bacteriology Research

  • Abbreviation: J. Bacteriol. Res.
  • Language: English
  • ISSN: 2006-9871
  • DOI: 10.5897/JBR
  • Start Year: 2009
  • Published Articles: 117

Full Length Research Paper

Amidase from plant growth promoting rhizobacterium

Sonia Chacko1, Pramod W. Ramteke2* and Suchit A. John2
1Department of Biotechnology, Allahabad Agricultural Institute – Deemed University, Allahabad – 211007, India. 2Department of Biological Sciences, Allahabad Agricultural Institute – Deemed University, Allahabad – 211007, India.
Email: [email protected]

  •  Accepted: 24 June 2009
  •  Published: 31 July 2009


Ninety three cultures of plant growth promoting rhizobacteria (PGPR) isolated from rhizosphere of Pisum sativum, among them one isolate identified asPseudomonas putida was found to be potential amidase producer. The organism exhibited a battery of PGPR traits including enhanced production of plant growth hormone indoleacetic acid (IAA) and siderophore. P. putida MTCC 6809 exhibited both intracellular and extra-cellular amidase activity. The organism produced maximum extracellular amidase enzyme at 30°C and pH 7.5 in shaking state. The organism hydrolyzed a wide range of aliphatic amides that included acetamide, propionamide, acrylamide and butyramide. Acrylamide is a known carcinogen, teratogen and neurotoxicant and utilization of acrylamide by P. putida MTCC 6809 assume great importance. The organism is also tolerant to number of heavy metals at higher levels. These characteristics make P. putida MTCC 6809 an excellent candidate for field application in contaminated soil.


Key words: PGPR, amidase, aliphatic amides, acrylamide, heavy metal tolerance.