African Journal of
Bacteriology Research

  • Abbreviation: J. Bacteriol. Res.
  • Language: English
  • ISSN: 2006-9871
  • DOI: 10.5897/JBR
  • Start Year: 2009
  • Published Articles: 103

Full Length Research Paper

Evaluation of in-vitro inhibitory effect of honey on some microbial isolate

Raied Taha Al- Naama
Department of Microbiology, College of Dentistry, University of Basrah, Iraq.
Email: [email protected]

  • Article Number - EDC25D78969
  • Vol.1(6), pp. 064-067, September 2009
  •  Accepted: 24 June 2009
  •  Published: 30 September 2009

Abstract

The aim of this study was to investigate the antimicrobial activity of honey sample from Basrah region against certain microbial isolate. Different concentrations (25.0, 50.0, 75.0 and 100.0%) of honey sample where checked for their antimicrobial activities, using some medically important micro-organisms includingEscherichia coli, Pseudomonas spp. and Staphylococcus aureus. The minimum inhibitory concentrations (MIC) of the honey sample were determined on the selected micro-organisms by using broth dilution technique. The sample of honey show inhibitory effect in vitro at 50, 75 and 100% concentration on the various investigated micro-organism except at 50% concentration where there was no inhibition zone on S. aureus. However, no effect was observed at 25% concentration. The MIC for E. coli, Pseudomonas spp. and S. aureus were 6.25, 1.5 and 12.5 mg/ml respectively. The study shows that honey, like antibiotics, has certain organisms sensitive to it, and provides alternative therapy against certain bacteria and is also shown to have antimicrobial action against a broad spectrum of bacteria (both gram-positive and -negative bacteria).

 

Key words: Staphylococcus aureusEscherichia coliPseudomonas aeruginosa, honey, antibiotics, sensitivity, antimicrobial.

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