Journal of
Cell and Animal Biology

  • Abbreviation: J. Cell Anim. Biol.
  • Language: English
  • ISSN: 1996-0867
  • DOI: 10.5897/JCAB
  • Start Year: 2007
  • Published Articles: 261

Study on ultrastructure of Leishmania major and Lizard leishmania

Bahram Kazemi1, 2*, Mohammad Hassan Heidari1, 3, Mehdi Naderi2, Abbas Piryaei 1, 3 and Mohammad -Reza Nazari -Pouya2
1Cellular and Molecular Biology Research Center -Shahid Beheshti University M. C., Tehran, I. R. Iran. 2Parasitology Department - Shahid Beheshti University, M. C., Tehran, I. R. Iran. 3Anatomy Department - Shahid Beheshti University M. C., Tehran, I. R. Iran.
Email: [email protected] and [email protected]

  •  Accepted: 16 May 2008
  •  Published: 30 June 2008

Abstract

We compared ultrastructure of Lizard Leishmania and Leishmania majorpromastigotes by electron microscope. Leishmania major (MRHO/R64/Nadim1) and Lizard leishmania promastigotes were mass cultured, collected by centrifugation and washed by PBS buffer three times, the ultrastructural features of both Leishmania promastigotes were observed by transmission electron microscope. Their subpellicular microtubules diameter and cytoplasmic membrane thickness are identical, but there is difference in two parasites in respect of distance between kinetoplast and flagella. We could not find golgi apparatus in Lizard Leishmania.

 

Key words: Lizard LeishmaniaLeishmania major, Transmission Electron Microscope.