Journal of
Cell and Animal Biology

  • Abbreviation: J. Cell Anim. Biol.
  • Language: English
  • ISSN: 1996-0867
  • DOI: 10.5897/JCAB
  • Start Year: 2007
  • Published Articles: 260

Full Length Research Paper

Effect of aqueous ozone solution on pancreatic cells

Al Hajjar Nadim1, Pitu Flaviu1, Nicodim Fiţ2, Pitu Florina1, Popa Calin1, Suarăsan Ilie3 and Emoke Pall2*
1University of Medicine and Pharmacy Cluj-Napoca, Institute of Gastroenterology-Hepatology ”Prof. O. Fodor”, Romania. 2University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine 3-5, Manastur Street, 400372, Cluj-Napoca, Romania. 3Technical University of Cluj-Napoca, Romania.
Email: [email protected]

  •  Accepted: 19 January 2012
  •  Published: 31 January 2012

Abstract

The aim of this study was to evaluate the effects of ozone solutions on the viability of pancreatic cells. The ozone was generated by an electrical discharge on high-purity oxygen molecules. Ozonation of the water was performed by bubbling ozone through sterile distilled water (O3 concentration 4 g/1000 L) and saline solution (O3 concentration 5 g/1000 L). After initial dilution of test solutions, serial dilutions were made. In total, seven were tested for both types of solutions (0.00312 mg O3/1000 L, 0.00625 mgO3/1000 L, 0.0125 mg O3/1000 L, 0.025 mg O3/1000 L, 0.05 mg O3/1000 L, 0.1 mg O3/1000 L and 0.2 mg O3/1000 L). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra-zoliumbromide (MTT) cytotoxicity test was performed in triplicate after 24 h of treatment.

 

Key words: Ozone, acute pancreatitis, aqueous solution, bactericidal effect.