Abstract

The aim of this study was to evaluate the effects of ozone solutions on the viability of pancreatic cells. The ozone was generated by an electrical discharge on high-purity oxygen molecules. Ozonation of the water was performed by bubbling ozone through sterile distilled water (O3 concentration 4 g/1000 L) and saline solution (O3 concentration 5 g/1000 L). After initial dilution of test solutions, serial dilutions were made. In total, seven were tested for both types of solutions (0.00312 mg O3/1000 L, 0.00625 mgO3/1000 L, 0.0125 mg O3/1000 L, 0.025 mg O3/1000 L, 0.05 mg O3/1000 L, 0.1 mg O3/1000 L and 0.2 mg O3/1000 L). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra-zoliumbromide (MTT) cytotoxicity test was performed in triplicate after 24 h of treatment.

Key words: Ozone, acute pancreatitis, aqueous solution, bactericidal effect.