Journal of
Cell and Animal Biology

  • Abbreviation: J. Cell Anim. Biol.
  • Language: English
  • ISSN: 1996-0867
  • DOI: 10.5897/JCAB
  • Start Year: 2007
  • Published Articles: 260

Full Length Research Paper

Dynamics of insecticide resistance and exploring biochemical mechanisms involved in pyrethroids and dichlorodiphenyltrichloroethane (DDT) cross-resistance in Anopheles gambiae s.l populations from Benin, West Africa

Nazaire Aïzoun*
  • Nazaire Aïzoun*
  • Centre de Recherche Entomologique de Cotonou (CREC), 06 BP 2604, Cotonou, Bénin; Faculté des Sciences et Techniques, Université d’Abomey Calavi, Calavi, Bénin.
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Roseric Azondekon
  • Roseric Azondekon
  • Centre de Recherche Entomologique de Cotonou (CREC), 06 BP 2604, Cotonou, Bénin; University of Massachusetts Amherst, Amherst, Massachusetts, USA.
  • Google Scholar
Rock Aïkpon
  • Rock Aïkpon
  • Centre de Recherche Entomologique de Cotonou (CREC), 06 BP 2604, Cotonou, Bénin; Faculté des Sciences et Techniques, Université d’Abomey Calavi, Calavi, Bénin.
  • Google Scholar
Rodrigue Anagonou
  • Rodrigue Anagonou
  • Centre de Recherche Entomologique de Cotonou (CREC), 06 BP 2604, Cotonou, Bénin; Faculté des Sciences et Techniques, Université d’Abomey Calavi, Calavi, Bénin.
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Virgile Gnanguenon
  • Virgile Gnanguenon
  • Centre de Recherche Entomologique de Cotonou (CREC), 06 BP 2604, Cotonou, Bénin; Faculté des Sciences et Techniques, Université d’Abomey Calavi, Calavi, Bénin.
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Martin Akogbéto
  • Martin Akogbéto
  • Centre de Recherche Entomologique de Cotonou (CREC), 06 BP 2604, Cotonou, Bénin; Faculté des Sciences et Techniques, Université d’Abomey Calavi, Calavi, Bénin.
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  •  Accepted: 07 February 2014
  •  Published: 31 March 2014

Abstract

As a result of the free-insecticide treated net distribution by National Malaria Control Programme (NMCP) in July 2011 throughout the entire country, it is useful to investigate the dynamics of insecticide resistance in Anopheles gambiae from Benin and the metabolic resistance mechanisms involved in this evolution of resistance. Larvae and pupae of A. gambiae s.l. mosquitoes were collected from the breeding sites in Littoral, Ouemé and Zou departments. Centers for Disease Control and Prevention (CDC), bioassays were performed with permethrin (21.5 µg per bottle) and dichlorodiphenyltrichloroethane (DDT) (100 µg per bottle) whereas World Health Organization (WHO) susceptibility tests were performed with lambdacyhalothrin (0.05%). CDC biochemical assays using synergists piperonyl butoxide (PBO) and ethacrynic acid (ETAA) were also carried out. A. gambiae Akron, Suru-léré and Bamè populations were resistant to permethrin and DDT in 2008 and 2013. A. gambiae Akron and Suru-léré were resistant to lambdacyhalothrin in 2013. A. gambiae Akron were susceptible to this product in 2008 whereas lambdacyhalothrin resistance status in A. gambiae Suru-léré populations in 2008 required further investigation. Mono-oxygenases were involved in resistance of A. gambiae Suru-léré to pyrethroids whereas glutathione S-transferases (GSTs) may play a role in A. gambiae Akron and Suru-léré resistant to DDT. This study shows that metabolic resistance conferred by detoxifying enzymes is an indication of phenotypic resistance to both DDT and pyrethroids in Southern Benin.

Key words: Dynamics, piperonyl butoxide, ethacrynic acid, insecticide, vectors, resistance.