Journal of
Cell and Animal Biology

  • Abbreviation: J. Cell Anim. Biol.
  • Language: English
  • ISSN: 1996-0867
  • DOI: 10.5897/JCAB
  • Start Year: 2007
  • Published Articles: 260

Full Length Research Paper

Genotyping of Toxoplasma gondii samples from Dakar

Amadou Ndiaye1, Pape Mbacké Sembene3, Ngor Faye3, Daouda Ndiaye4, Daniel Ajzenberg5, Niama Diop Sall1,2 and Ben Sikina Toguebaye3
1Department of Laboratories Abass NDAO Hospital, Dakar, Senegal. 2Faculty of Medicine, Pharmacy and Dentistry (FMPOS), Dakar, Laboratory of Medical Biochemistry., Dakar, Senegal 3Department of Animal Biology, Faculty of Science and Technology, Dakar – Senegal 4Department of Parasitology and Mycology Pharmacy and Dentistry (FMPOS), Faculty of Medicine, Dakar, Senegal. 5National Reference Center for Toxoplasmosis, Laboratory of Parasitology and Mycology, Centre Hospitalier - Universitaire Dupuytren, 2 avenue Martin Luther King, 87042 Limoges, France.
Email: [email protected], [email protected]

  •  Accepted: 14 November 2013
  •  Published: 31 December 2013

Abstract

Toxoplasmosis is an anthropozoonosis of medical and veterinary importance, caused by the protozoan Toxoplasma gondii. Oocysts shed by felids play a key role in parasite transmission as they contaminate meat-producing animals, vegetables and water consumed later by humans. In this work, we aimed to identify T. gondii isolated in Dakar (Senegal). The modified multiplex PCR technique based on length polymorphism of 15 microsatellite markers was used on a total of 10 isolates. The study shows that stem called Africa 1 or Type I / III (90%) and Africa 2 or Type I / II / III (10%) circulate in Dakar. Toxoplasma isolates and DNA extraction for genotyping analysis was performed directly on clinical samples for 56 patients and indirectly on infected mouse tissue (brain or ascitic fluid) or infected cell cultures after inoculation of clinical samples for 32 patients. These first results need to be followed by more extensive investigations.

 

Key words: Genotype, toxoplasmosis, Toxoplasma gondii.