Journal of
Clinical Pathology and Forensic Medicine

  • Abbreviation: J. Clin. Pathol. Forensic Med.
  • Language: English
  • ISSN: 2141-2405
  • DOI: 10.5897/JCPFM
  • Start Year: 2010
  • Published Articles: 29

Full Length Research Paper

Neuronal alterations in the prefrontal cortex of rats with carbon tetrachloride (CCl4) induced hepatic damage

Adedayo Damilare Adekomi1,2*, Olusola Bolaji Adewale3, Ola Adulrasheed Muhameed4, Adedoyin Mojeed Agbaje5 and Tajudeen Oyesina Oyesomi5
1Department of Anatomy, College of Medicine and Health Sciences, Afe Babalola University, Ado Ekiti, Ekiti State, Nigeria. 2Department of Anatomy and Cell Biology, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria. 3Department of Chemical Sciences, Biochemistry Unit, Afe Babalola University, Ado Ekiti, Ekiti State, Nigeria. 4Histopathology Unit, Pathology Department, University of Ilorin Teaching Hospital, Ilorin, Nigeria 5Department of Anatomy, Ekiti State University, Ado Ekiti, Nigeria.
Email: [email protected]

  •  Accepted: 05 July 2013
  •  Published: 31 August 2013


In cirrhosis, some toxic substances accumulate in the brain and modify its functional integrity. In this study, we investigate the impacts of liver damage on the neuronal profile of the prefrontal cortex (PFC) in a rat model of hepatic damage induced with carbon tetrachloride (CCl4). This study also evaluated the possible role of liver dysfunction in the etiology of neurodegenerative characteristics associated with the PFC. Ten male Wistar rats weighing 120 to 190 g body weight were used for this study. The rats were divided into 2 groups (A and B) of 5 rats each. The rats in group A (control group) were treated with phosphate buffered saline (PBS) solution only while the rats in group B (treatment group) were treated with carbon tetrachloride (CCl4). The prefrontal cortices of the rats were excised from skulls of the rats, fixed in formol calcium, while the livers were excised from the abdomen of the rats and were fixed in formol saline for cytoarchitectural study using Cresyl fast violet and hematoxylin and eosin stains respectively. The main neuropathological findings observed in this study include cortical necrosis, uneven neuronal loss with varying range of vacuolations in the prefrontal cortices of the CCl4 treated rats when compared with the PBS treated rats. It was observed that the administration of CCl4 induces changes in hepatocellular morphology of the treated rats and these include moderate vascular congestion and extensive cytoplasmic damage in the hepatocytes. These results could be due to loss of hepatic functions.


Key words: Liver damage, neuropathology, neurons, prefrontal cortex, rat.