Journal of
Developmental Biology and Tissue Engineering

  • Abbreviation: J. Dev. Biol. Tissue Eng.
  • Language: English
  • ISSN: 2141-2251
  • DOI: 10.5897/JDBTE
  • Start Year: 2009
  • Published Articles: 42

Full Length Research Paper

Ultrasonic analyses algorithm on an ex vivo produced oral mucosal equivalent

Frank Winterroth1*, Junho Lee2, Shiuhyang Kuo3, J. Brian Fowlkes1,4, Stephen E. Feinberg1,3, Scott J. Hollister1 and Kyle W. Hollman1,5
1Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA. 2School of Dentistry, University of Michigan, Ann Arbor, MI, USA. 3Department of Oral and Maxillofacial Surgery, University of Michigan, Ann Arbor, MI, USA. 4Department of Radiology, University of Michigan, Ann Arbor, MI, USA. 5Sound Sight Research, Livonia, MI, USA.
Email: [email protected]

  •  Accepted: 12 August 2011
  •  Published: 31 August 2011


This study examines the use of high-resolution ultrasound with an analyses algorithm to accurately monitor development of an ex vivo produced oral mucosal equivalent (EVPOME). We used ultrasonic profilometry to examine EVPOME development as seeded cells on its surface proliferate. As these engineered structures develop, seeded cells stratify from their differentiation and produce a keratinized protective upper layer. Some of these transformations could alter backscatter of ultrasonic signals and produce scattering of the signal similar to an unseeded scaffold. Developing non-invasive in vitro ultrasonic monitoring allows adjusting tissue cultivation in-process, accounting for biological variations in the development of the EVPOME.

Key words: Acoustic microscopy, oral mucosa, non-invasive assessment, tissue engineering, ultrasound.