Journal of
Developmental Biology and Tissue Engineering

  • Abbreviation: J. Dev. Biol. Tissue Eng.
  • Language: English
  • ISSN: 2141-2251
  • DOI: 10.5897/JDBTE
  • Start Year: 2009
  • Published Articles: 42

Full Length Research Paper

A study on the formation and development of Panax bipinnatifidus Seem. adventitious root

Bui Dinh Thach
  • Bui Dinh Thach
  • Institute of Tropical Biology, Vietnam Academy of Science and Technology, Vietnam.
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Lenguyen Tu Linh
  • Lenguyen Tu Linh
  • Institute of Tropical Biology, Vietnam Academy of Science and Technology, Vietnam.
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Nguyen ThiThuy Van
  • Nguyen ThiThuy Van
  • Institute of Tropical Biology, Vietnam Academy of Science and Technology, Vietnam.
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Trinh Thi Ben
  • Trinh Thi Ben
  • Institute of Tropical Biology, Vietnam Academy of Science and Technology, Vietnam.
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Mai Truong
  • Mai Truong
  • Institute of Tropical Biology, Vietnam Academy of Science and Technology, Vietnam.
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Nguyen Huu Ho
  • Nguyen Huu Ho
  • Institute of Tropical Biology, Vietnam Academy of Science and Technology, Vietnam.
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Bui Dinh Thach
  • Bui Dinh Thach
  • Institute of Tropical Biology, Vietnam Academy of Science and Technology, Vietnam.
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Lenguyen Tu Linh
  • Lenguyen Tu Linh
  • Institute of Tropical Biology, Vietnam Academy of Science and Technology, Vietnam.
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Nguyen ThiThuy Van
  • Nguyen ThiThuy Van
  • Institute of Tropical Biology, Vietnam Academy of Science and Technology, Vietnam.
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Trinh Thi Ben
  • Trinh Thi Ben
  • Institute of Tropical Biology, Vietnam Academy of Science and Technology, Vietnam.
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Mai Truong
  • Mai Truong
  • Institute of Tropical Biology, Vietnam Academy of Science and Technology, Vietnam.
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Nguyen Huu Ho
  • Nguyen Huu Ho
  • Institute of Tropical Biology, Vietnam Academy of Science and Technology, Vietnam.
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  •  Received: 30 June 2014
  •  Accepted: 11 July 2014
  •  Published: 31 July 2014

Abstract

This study aimed to generate adventitious roots from root and stem-derived callus of Panax bipinnatifidus Seem. Callus formation was observed best (83.3%) in MS media supplemented with 3.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1 mg/L indolebutyric acid (IBA). Callus generated adventitious roots in MS media supplemented with 2.0 mg/L IBA at high frequency (66.7%). The optimal culture condition for the development of adventitious roots was liquid MS media supplemented with 40 mg/L sucrose and maintained at pH 6.0. 

 

Key words: Adventitious root, stem, basic MS media.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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