This study aimed to generate adventitious roots from root and stem-derived callus of Panax bipinnatifidus Seem. Callus formation was observed best (83.3%) in MS media supplemented with 3.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1 mg/L indolebutyric acid (IBA). Callus generated adventitious roots in MS media supplemented with 2.0 mg/L IBA at high frequency (66.7%). The optimal culture condition for the development of adventitious roots was liquid MS media supplemented with 40 mg/L sucrose and maintained at pH 6.0.
Key words: Adventitious root, stem, basic MS media.
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