Journal of
Developmental Biology and Tissue Engineering

  • Abbreviation: J. Dev. Biol. Tissue Eng.
  • Language: English
  • ISSN: 2141-2251
  • DOI: 10.5897/JDBTE
  • Start Year: 2009
  • Published Articles: 42

Full Length Research Paper

Genistein, the phytoestrogen induces heart-and-soul (has) phenotypes in zebrafish embryo

Antony Bakkiyanathan, Asha Mary Joseph, Loganathan Tharani and Raghunathan Malathi*
Department of Genetics, Dr ALM PG IBMS, University of Madras, Taramani, Chennai, India.
Email: [email protected]

  •  Accepted: 02 March 2010
  •  Published: 31 December 2010


Zebrafish (Danio rerio) embryos treated with a small plant molecule, genistein generated a mimic of zebrafish VEGF-A loss of function model. These embryos develop with an enlarged pericardium and major blood vessel deficiencies and their morphological assessment after 48 h post fertilization indicates a nearly complete absence of both axial and intersegmental vasculature with no or reduced numbers of circulating red blood cells resulting in distinctive phenotype of “heart and soul”. Thus, zebrafish provides an excellent vertebrate model system to study developmental process and identify novel genes involved during development.

Key words: Genistein, VEGF, has, vertebrate development, zebrafish, angiogenesis