An efficient and rapid regeneration protocol was developed using embryo from germinating seedlings of cultivar of cotton (var.narishima) as an explant. A vertical slit given in the apex of embryo shoot regeneration was successful on murashige and skoog medium with 2 mg/L benzyl amino purine (BAP). The shoots were sub cultured in every 15 days in regeneration medium and later they were transferred to rooting medium (1 mg/L IBA).
Key words: Cotton, embryo, tissue culture, regeneration, plant growth regulators.
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