Journal of
Developmental Biology and Tissue Engineering

  • Abbreviation: J. Dev. Biol. Tissue Eng.
  • Language: English
  • ISSN: 2141-2251
  • DOI: 10.5897/JDBTE
  • Start Year: 2009
  • Published Articles: 42

Full Length Research Paper

Optimization of medium conditions for efficient plant regeneration from embryo of cotton (var.narishima)

M. Guru Prasad, P. Sudhakar and T. N. V. K. V. Prasad*
Institute of Frontier Technology, Regional Agricultural Research Station, Acharya N G Ranga Agricultural University, Tirupati,-517 502, A. P., India.
Email: [email protected]

  •  Accepted: 13 January 2011
  •  Published: 27 February 2011


An efficient and rapid regeneration protocol was developed using embryo from germinating seedlings of cultivar of cotton (var.narishima) as an explant. A vertical slit given in the apex of embryo shoot regeneration was successful on murashige and skoog medium with 2 mg/L benzyl amino purine (BAP). The shoots were sub cultured in every 15 days in regeneration medium and later they were transferred to rooting medium (1 mg/L IBA). 

Key words: Cotton, embryo, tissue culture, regeneration, plant growth regulators.