Journal of
Developmental Biology and Tissue Engineering

  • Abbreviation: J. Dev. Biol. Tissue Eng.
  • Language: English
  • ISSN: 2141-2251
  • DOI: 10.5897/JDBTE
  • Start Year: 2009
  • Published Articles: 42

Full Length Research Paper

Fibrin sealant as scaffold can be a suitable substitute to autograft in short peripheral nerve defect in rats

Z. Jamalpoor1,5, M. Ebrahimi2,6, N. Amirizadeh3, K. Mansoori4, A. Asgari5 and M. R. Nourani1,2*
1Trauma Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. 2Chemical Injury Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. 3Iranian Blood Transfusion Organization, Tehran, Iran. 4Department of Physical Medicine and Rehabilitation, Shafayahyaian Rehabilitation Hospital, Iran University of Medical Sciences, Tehran, Iran. 5Department of Physiology, Baqiyatallah University of Medical Sciences, Tehran, Iran. 6Department of Organ Anatomy, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Email: [email protected]

  •  Accepted: 22 April 2011
  •  Published: 30 June 2011


There is considerable evidence that peripheral nerves have the potential to regenerate in an appropriate microenvironment. In this study, the process of nerve regeneration through a fibrin scaffold was examined. 45 male Wistar rats were randomly divided into one control, Autograft (Auto) and two experimental groups, Epineurum (Epi) and Fibrin scaffold (Fib). Right sciatic nerve was exposed of which 5 mm was cut. The nerve defect was then bridged with a nerve autograft, empty epineurium and fibrin scaffold in the corresponding groups. All animals were examined one, three and five weeks after the operation to evaluate nerve regeneration and functional recovery employing light microscopy and walking track analysis, respectively. The gastrocnemius muscle contractility was also examined at 35th day post surgery in all groups using electromyography (EMG). Histological, functional evaluation and EMG evidences show that the nerve regeneration in both groups Auto and Fib were statistically equivalent  and superior to that of Epi group (P<0.01). The present findings indicate that a fibrin scaffold enhances nerve regeneration as effective as a nerve autograft.


Key words: Sciatic nerve, fibrin scaffold, electromyography, autograft.