Cells from legs of Gastrimargus musicus, a grey brown grasshopper were grown in simple media. The medium was tested for growth of muscle cells and with supplements. It was found that the medium containing calcium carbonate had round cells growing close to each other in clumps. The medium containing creatine had cells growing which were elongated and branched and had all the features of fully developed muscle tissue. The muscle tissues grew to a size, more than double that of the original grasshopper and were maintained in the medium supplemented with creatine for more than two months.
Key words: Gastrimargus musicus, cell culture medium, insect cell culture, muscle
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